Project description:Mycoplasmas are wall-less parasitic bacteria possessing extremely small genomes. Despite this, growth rates differ among mycoplasma species with doubling times ranging from only 0.5h to 16h. Here, we analyzed the whole proteomes of M. hyopneumoniae and M. feriruminatoris and performed a comparative study in slow and fast growing mycoplasmas.
Project description:The goal of this experiment is to determine the overall relative strength of promoter sequences in Mycoplasma feriruminatoris. For this, 2 replicates were grown in parallel in Hayflick media and the RNA wa extracted at exponential growth phase (20 hours). With this data, new promoter sequences could be designed and further validated by the use of RT-qPCR and reporter assays.
