Project description:This is an oligonucleotide-based proof-of-concept study for a method to detect 5-hydroxymethyluracil (5hmU) in a single base-resolution on NGS platform. The method will be useful to identify the effect of 5hmU to genome functions.
Project description:Proof-of-concept of a new method involving the limited digestion and subsequent ligation of intramolecular RNA structures in situ followed by deep sequencing Proof-of-concept of RPL in S. cerevisiae and H. sapiens tissue culture
Project description:In this study, we report a novel sDNA sequence can specifically target P53 protein. As a proof of concept, we derivatized this new sDNA fragment and attached biotin at its 5'end. using chemical proteome methods, we explored the new sDNA enrichment ability of P53 protein.
