Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional start site mapping of P14 WT and P14 Tbpl2-/- mutant mouse oocytes

ABSTRACT: Transcriptional start site mapping of P14 WT and P14 Tbpl2-/- mutant mouse oocytes

PROVIDER: PRJEB37152 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional start site mapping of P14 WT and P14 Tbpl2-/- mutant mouse oocytes

Project description:We used SLIC-CAGE to map transcriptional start sites (TSSs) of P14 WT and P14 Tbpl2-/- mutant mouse oocytes. Comparison of WT and Tbpl2-/- oocytes demonstrates that Tbpl2 guides transcriptional start site selection in the growing oocyte. This TSS selection is different compared to the canonical somatic type of TSS selection and depends on TATA-like elements as core promoter motifs, recognised by Tbpl2.

2020-11-12 | E-MTAB-8866 | biostudies-arrayexpress

SLIC-CAGE (Super-Low Input Carrier-CAGE) transcription start site mapping of mouse migratory and gonadal primordial germ cells, postnatal oocytes and early embryo

Project description:We used SLIC-CAGE to map transcription start sites (TSSs) of mouse primordial germ cells from embryonic days 9.5-16.5, postnatal oocytes (P6, P14 and MII), and early 2-cell and 4-cell mouse embryos. We use this TSS data to show that the mouse germline development starts with the somatic promoter code with a prominent switch to the maternal code (W-box dependent) occurring during the follicular oogenesis. We also find that the promoters of gonadal germ cells are characterised by a previously unknown divergence from the somatic transcription initiation. This divergence is distinct from the promoter code used later by the developing oocytes and reveals genome-wide promoter remodelling during early female and male germline development.

2022-02-14 | E-MTAB-9757 | biostudies-arrayexpress

Transcription Start Site (TSS) mapping and total RNAseq of Streptomyces scabies wild-type (WT) versus Streptomyces scabies ΔbtmL

Project description:The aim of this experiment was to map the transcription start sites (TSSs) in the bottromycin biosynthetic gene cluster from Streptomyces scabies, qualitatively assess the expression levels of this cluster within the bacterium's transcriptome and evaluate whether deletion of a potential regulatory gene in the cluster, btmL affects gene cluster expression.

2020-02-19 | E-MTAB-8236 | biostudies-arrayexpress

RNA-Seq from wt oocytes

Project description:To determine gene expression in mouse ovulated oocytes 3 Replicates of litters of wild type ovulated oocytes

2012-06-18 | E-GEOD-33922 | biostudies-arrayexpress

Genome-wide transcriptional start site mapping and sRNA identification in the pathogen Leptospira interrogans

Project description:Leptospira are emerging zoonotic pathogens transmitted from animals to humans typically through contaminated environmental sources of water and soil. Transcriptional regulation of pathogenic Leptospira spp. underlying the adaptive response to different hosts and environmental conditions remains elusive. In this study, we provide the first global Transcriptional Start Site (TSS) map of a Leptospira species. RNA was obtained from the pathogen Leptospira interrogans grown at 30° (optimal in vitro temperature) and 37°C (host temperature) and selectively enriched for 5' ends of native transcripts. Primary TSS (pTSS) was identified for 2,865 genes, accounting for 67% of the total genome. The majority of the TSSs were located between 0 to 10 nucleotides from the translational start site. Comparative dRNA-seq analysis revealed conservation of most pTSS at 30° and 37°C. Promoter prediction algorithms allow the identification of the binding sites of the alternative sigma factor sigma 54. However, other motifs were not identified indicating that Leptospira consensus promoter sequences are inherently different from the E. coli model. RNA sequencing also identified 277 and 226 putative small regulatory RNAs (sRNAs) at 30°C and 37°C, respectively, including 8 validated sRNAs by Northern blots. These results provide the first global view of transcriptional start sites and the repertoire of sRNAs in L. interrogans, and will establish a foundation for future experimental work on gene regulation under various environmental conditions including those in the host.

2017-01-23 | GSE92976 | GEO

Comparison of transcriptomes between Marf1+/+ (WT) and Marf1 ENU/ENU (Mutant) fully-grown oocytes (FGO)

Project description:Marf1 (MUT) female mice are infertile and the meiosis of the oocytes are arrest at prophase I. We thought to identify the potential causes of the meiotic arrest phenotype in the mutant oocytes by comparing the transcriptomes of the WT and mutant fully-grown oocytes (from 23-d old mice) that are transcriptional silent. We compared the transcriptomes of WT and MUT FGOs.

2012-03-31 | GSE31985 | GEO

Transcription start site identification in Bacillus subtilis

Project description:Cappable-seq was used to map transcription start sites globally in wild type Bacillus subtilis. Total RNA was isolated from cells grown in LB media until exponential phase. RNA corresponding to transcription start sites was capped with a 5' biotin tag, which was used for enrichment via a pull down with streptavidin beads. Enriched RNA was converted to cDNA and then subjected to illumina sequencing.

2021-04-16 | E-MTAB-8582 | biostudies-arrayexpress

RNA-seq of P14 mouse brain, WT and PRMT5 olig2-cre KO

Project description:Analysis of the transcriptiome of p14 mouse brains to investigate the contribution of PRMT5 in oligodendrocyte gene expression and alternative splicing regulation

2018-08-01 | GSE116242 | GEO

RNA-Seq from wt oocytes

Project description:To determine gene expression in mouse ovulated oocytes

2012-06-18 | GSE33922 | GEO

Comparison of transcriptomes between Marf1+/+ (WT) and Marf1 ENU/ENU (Mutant) fully-grown oocytes (FGO)

2012-03-30 | E-GEOD-31985 | biostudies-arrayexpress