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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

ABSTRACT: The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

PROVIDER: PRJEB37814 | ENA |

REPOSITORIES: ENA

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Similar Datasets

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) ) on the transcriptome of CD44-overexpressing IMR90 cells.

2020-04-25 | E-MTAB-8943 | biostudies-arrayexpress

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

Project description:The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

| PRJNA480072 | ENA

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) and partially fragmented NSF-HA (fNSF-HA) on the transcriptome of IMR90 cells. Related RNA-Seq datasets are deposited in ArrayExpress (E-MTAB-8943).

2019-01-01 | GSE116759 | GEO

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44 protein-protein interactions.

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) and partially fragmented NSF-HA (fNSF-HA) on CD44 protein-protein interactions.

2020-03-24 | PXD013445 | Pride

The role of CD44 in human fibroblasts IMR90 cells

Project description:The role of CD44 in human fibroblasts IMR90 cells were evaluated by comparing the transcriptome of control and CD44-knockdown IMR90 cells.

2023-05-31 | E-MTAB-12936 | biostudies-arrayexpress

Hyaluronan-CD44 signaling promotes pro-tumor inflammation in breast cancer

Project description:Hyaluronan-CD44 signaling promotes pro-tumor inflammation in breast cancer

| PRJNA625295 | ENA

E-MTAB-8943

Project description:The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

2021-03-22 | E-MTAB-8943 | ExpressionAtlas

Expression data from CD44-knockdown MDA-MB-231 human breast cancer cells.

Project description:CD44, an adhesion molecule that binds to extracellular matrix, primarily to hyaluronan (HA), has been implicated in cancer cell migration, invasion, and metastasis. CD44 has also recently been recognized as a marker for stem cells of several types of cancer. However, the roles of CD44 in the development of bone metastasis still remain unclear. To explore this issue, we established the MDA-MB-231 human breast cancer cells stably expressing short hairpin RNA against CD44. The CD44-knockdown MDA-MB-231 cells (MDA-MB-231 shCD44-2 and shCD44-3) were analyzed. As control, MDA-MB-231 cells stably expressing shRNA against firefly luciferase (shLuc) were used. Total of three samples. No replicates.

2013-04-24 | E-GEOD-45613 | biostudies-arrayexpress

Expression data from CD44-knockdown MDA-MB-231 human breast cancer cells.

2013-04-24 | GSE45613 | GEO

Hyaluronan-CD44 signaling promotes pro-tumor inflammation in breast cancer

Project description:We report the application of next-generation sequencing (NGS) technology for high-throughput profiling of RNA expression in human breast cancer cells. The goal of this study was to compare NGS-derived transcriptome profiling (RNA-seq) from cells expressing either wildtype or knockout CD44 to identify key downstream signaling events of HA-CD44 interactions. Methods: This experiment was repeated for a total of three times and replicate RNA samples were extracted and submitted for Illumina Sequencing. Twelve dual-indexed Illumina TruSeq stranded mRNA libraries were pooled for sequencing on 1 lane of a HiSeq 2500 using v4 chemistry to generate 2x50 bp paired-end reads. After applying the Benjamini-Hochberg multiple hypothesis adjustment to raw p-values, differentially expressed genes were identified requiring that they had a log2 fold change > 0 and an adjusted p-value ≤ 0.01. Results: A total of 1,530 and 320 genes were impacted by CD44 KO in the Hs578T cells and MDA-MB-231 cells, respectively. When comparing each of these data sets, 40 genes were differentially expressed in both Hs578T and MDA-MB-231 cells upon deletion of CD44. Conclusions: Gene ontology (GO) analysis revealed that CD44 KO in breast cancer cells altered expression of genes involved in both cell adhesion and cytokine activity. This study provides a framework for the application of NGS to compare differential gene expression between genetically edited mammalian cell populations.

2021-03-29 | GSE148657 | GEO

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