Project description:Gradient fractions of RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms. RNAi was induced using tetracycline and cell extracts were fractionated into polysomal and monosome-non-ribosome-associated fractions.

2017-12-16 | E-MTAB-6239 | biostudies-arrayexpress

RNAi of TbNMD3 in procyclic form Trypanosoma brucei lines

Project description:A procyclic form Trypansome brucei RNAi line (PTT parental line, transfected with pALC14 incorporating a TbNMD3 gene fragment) capable of inducing depletion of TbNMD3 was analysed for mRNA expression by RNAseq Cells were grown for 72 hours in culture; RNAi was induced in cells by the addition of 1 microgram/ml of tetracycline

2015-04-28 | E-GEOD-67246 | biostudies-arrayexpress

In vivo RNA interference RNAi target sequencing of Trypanosoma brucei

Project description:A direct comparison of RNAi in vitro with RNAi in vivo is being performed using RNA interference (RNAi) target sequencing (RIT-Seq) of Trypanosoma brucei to identify all genes specifically required for growth in vivo (the infectome). Assembly of the bloodstream-form T. brucei RNAi library and the RNAi target sequencing (RIT-seq) approach in African trypanosomes were reported previously in Alsford, S. et al. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res 21, 915-924, 264 doi:gr.115089.110 [pii] 265 10.1101/gr.115089.110 (2011) and Alsford,S et al. High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232236 doi:10.1038/nature10771 (2012). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-01-21 | E-ERAD-110 | biostudies-arrayexpress

RNA-seq of Trypanosoma brucei brucei ZC3H22 RNAi depletion in procyclic forms

Project description:RNA-seq of Trypanosoma brucei brucei ZC3H22 RNAi depletion in procyclic forms

| PRJEB40988 | ENA

RNAi targeting ZC3H20 in differentiating EATRO1125 Trypanosoma brucei

Project description:RNAi targeting ZC3H20 in differentiating EATRO1125 Trypanosoma brucei

| PRJEB32720 | ENA

PUF3 RNAi in bloodstream-form of Trypanosoma brucei

Project description:PUF3 RNAi in bloodstream-form of Trypanosoma brucei

| PRJEB33062 | ENA