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ChIP-seq experiments of Estrogen Receptor Alpha (ERa) in the human breast cancer cell line T47D stably expressing GFP or neoGATA3

ABSTRACT: ChIP-seq experiments of Estrogen Receptor Alpha (ERa) in the human breast cancer cell line T47D stably expressing GFP or neoGATA3

PROVIDER: PRJEB38628 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4184101.fastq.gz	Fastqsanger.gz
	ERR4184102.fastq.gz	Fastqsanger.gz
	ERR4184103.fastq.gz	Fastqsanger.gz
	ERR4184104.fastq.gz	Fastqsanger.gz
	ERR4184105.fastq.gz	Fastqsanger.gz

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ChIP-seq experiments of Estrogen Receptor Alpha (ERa) in the human breast cancer cell line T47D stably expressing GFP or neoGATA3

Project description:ChIP-seq experiments of Estrogen Receptor Alpha (ERa) in the human breast cancer cell line T47D stably expressing GFP or neoGATA3

| PRJEB38628 | ENA

ChIP-seq experiments of Estrogen Receptor Alpha (ERa) in the human breast cancer cell line T47D stably expressing GFP or neoGATA3

Project description:In this study, we investigated the functions of the most common GATA3 mutation (X308_Splice) that we called “neoGATA3”. Analysis of available molecular data from >3000 breast cancer patients revealed a dysregulation of the ERa-dependent transcriptional response in tumors carrying this mutations. ChIP-Seq analysis indicated that ERa binding is reduced in neoGATA3-expressing cells, especially at distal regions, suggesting that neoGATA3 interferes with the fine-tuning of ERa-dependent gene expression.

2020-06-29 | E-MTAB-9121 | biostudies-arrayexpress

Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

Project description:Estrogen Receptor a (ERa) bindning to DNA was profiled by ChIP-seq in MCF-7 and T47D cells transduced with either control sgRNA, or sgRNA targeting a specific enhancer region (enhancer588). ERa in MCF-7 and T47D control or enhancer588-targeted cells

2015-12-14 | E-GEOD-75779 | biostudies-arrayexpress

Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

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ER/PR RIME Experiments in MCF7 and T47D cell lines

Project description:Immuno-precipitation followed by MS was performed using the RIME protocol in this study. Estrogen Receptor (ER) and Progestorone Receptor (PR) were targetted using antibodies. The experiments were performed in a quantitative manner using SILAC labelling. Cells grown in complete serum (estrogenic) conditions were compared against an cells in similar complete media, however supplemented with Progesterone (PG) or R5020 for 4 hours. Experiments were performed in MCF7 and T47D cell lines

2015-05-26 | PXD002104 | Pride

Transcription profiling of rat PC12 cells stably transfected with GFP, GFP-SH2-Bb, or GFP-SH2-Bb(R555E), treated with or without NGF for 6h

Project description:Expression profiling of PC12 cells stably transfected with GFP, GFP-SH2-Bb, or GFP-SH2-Bb(R555E), treated with or without NGF for 6h Experiment Overall Design: Total RNA was obtained from PC12 cells Experiment Overall Design: -stably transfected with GFP (G0) Experiment Overall Design: -stably transfected with GFP and treated for 6h with NGF (G6) Experiment Overall Design: -stably transfected with GFP-SH2-Bb (F0) Experiment Overall Design: -stably transfected with GFP-SH2-Bb and treated for 6h with NGF (F6) Experiment Overall Design: -stably transfected with GFP-SH2-Bb(R555E) (R0) Experiment Overall Design: -stably transfected with GFP-SH2-Bb(R555E) (R6)

2008-06-13 | E-GEOD-4557 | biostudies-arrayexpress

T47D shNT versus T47D shRON

Project description:T47D shNT versus T47D shRON

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T47D Breast cancer cell line ChIP-seq data

Project description:We performed genome-wide PADI2 ChIP seq experiments in T47D cell lines and also RNP2 ChIP seq in T47D cells only expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2.

2018-11-21 | GSE105793 | GEO

T47D cell line transfected with various ESR1 fusion genes (or with estrogen treatment), vs. YFP transfection alone

Project description:Gene expression profiling of the downstream transcriptional changes induced by ESR1 fusion genes observed in human breast tumors resistant to hormone therapy Experimental T47D cells stably expressing ESR1 fusion genes (or T47D cells treated with estradiol) vs. T47D cells expressing YFP

2015-06-12 | E-GEOD-69774 | biostudies-arrayexpress

RNA-seq of MCF10A cells stably expressing empty vector, GFP, miR-105, or MYC

Project description:To identify gene expression changes associated with overexpression of miR-105 or MYC in MCF10A non-cancerous human mammary epithelial cells, we analyzed RNA isolated from engineered MCF10A cell lines that stably express empty vector, GFP, miR-105, or MYC by RNA-seq. Gene expression in cells overexpressing miR-105 or MYC was compared to cells expressing the empty vector or GFP, both of which served as controls in this experiment.

2018-02-11 | GSE106502 | GEO

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