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Microbiome diversity within three manure-based small biogas plants

ABSTRACT: Microbiome diversity of three manure-based small biogas plants

PROVIDER: PRJEB38916 | ENA |

REPOSITORIES: ENA

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Microbiome Diversity and Community-Level Change Points within Manure-based small Biogas Plants.

Project description: Not available

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Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm

Project description:Meta-proteomics analysis approach in the application of biogas production from anaerobic digestion has many advantages that has not been fully uncovered yet. This study aims to investigate biogas production from a stable 2-stage chicken manure fermentation system in chemical and biological perspective. The diversity and functional protein changes from the 1st stage to 2nd stage is a good indication to expose the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explain the causation of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, the hydrogenotrophic methanogenic pathway is adopted as indicative of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomic analysis, this study could provide both species names of microorganism and enzymes to directly point the generation pathway of methane and carbon dioxide in investigating biogas production of chicken manure.

2021-02-08 | PXD022498 | Pride

Anaerobic biogas plants via LC-MS/MS

Project description:Anaerobic degradation (AD) of heterogeneous agricultural substrates is a complex process involving a diverse microbial community. While microbial community composition of a variety of biogas plants (BPs) is well described, little is known about metabolic processes and microbial interaction patterns. Here, we analyzed 16 large-scale BPs using metaproteomics. All metabolic steps of AD were observed in the metaproteome, and multivariate analyses indicated that they were shaped by temperature, pH, volatile fatty acid content and substrate types. Biogas plants can be subdivided into hydrogenotrophic, acetoclastic or a mixture of both methanogenic pathways based on their process parameters, taxonomic and functional metaproteome. Network analyses showed large differences in metabolic and microbial interaction patterns. Both, number of interactions and interaction partners were highly dependent on the prevalent methanogenic pathway for most species. Nevertheless, we observed a highly conserved metabolism of different abundant Pseudomonas spp. for all BPs indicating a key role during AD in carbohydrate hydrolysis irrespectively of variabilities in substrate input and process parameters. Thus, Pseudomonas spp. are of high importance for robust and versatile AD food webs, which highlight a large variety of downstream metabolic processes for their respective methanogenic pathways.

2019-11-11 | PXD014605 | Pride

Amplicon sequencing of four biogas plants

Project description:Amplicon sequencing of four biogas plants

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pig manure and biogas residue co-composting sample

Project description:pig manure and biogas residue co-composting sample Raw sequence reads

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Composting material of pig manure and biogas residue

Project description:Microbial community succession during pig manure and biogas residue co-composting

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Metagenome data of the four biogas plants of the "Biogas Marker" project

Project description:Metagenome data of the four biogas plants of the "Biogas Marker" project

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Effect of biogas slurry combined with biochar on soil microbial function gene

Project description:The functional diversity of soil microbial communities was explored for a poplar plantation, which was treated solely with biogas slurry, or combined with biochar at different fertilization intensities over several years.

2020-03-30 | GSE133800 | GEO

Metagenomic sequencing of two full scale manure co-digesting biogas plants

Project description:The Illumina HiSeq 2000 platform was used to sequence two metagenomes from two Danish biogas plants.

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Genomic hybridization of Methanobacteria to identify the diversity in strains on manure and olive mill waste.

Project description:Population dynamics of methanogenic genera was investigated in pilot anaerobic digesters. Cattle manure and two-phase olive mill wastes were codigested at a 3:1 ratio in two reactors operated at 37 ï¾°C and 55 ï¾°C. Other two reactors were run with either residue at 37 ï¾°C. Sludge DNA extracted from samples taken from all four reactors on days 4, 14 and 28 of digestion was used for hybridisation with the AnaeroChip, an oligonucleotide microarray targeting those groups of methanogenic archaea that are commonly found under mesophilic and thermophilic conditions (Franke-Whittle et al. 2009, in press, doi:10.1016/j.mimet.2009.09.017).

2010-11-10 | E-MEXP-2453 | biostudies-arrayexpress

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