ABSTRACT: The N. americanus-specific primers were designed based on the reference sequences complete mitochondrial genome sequences of this parasite available at GenBank (Acc. Nos. NC_003416, AJ556134, NW_013561619)
Project description:Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development.
Project description:In the current project we have carried out the first proteogenomic analysis of a parasitic helminth. This has allowed us to significantly improve the genome annotation and provide a robust characterisation of the ES proteome from the adult N. americanus. Our findings show important information on key families of proteins with both known and unknown functions which may be instrumental for host-parasite interactions, parasite survival and immune regulation. Description of these proteins is useful for informing future identification of vaccine and drug targets, diagnostics and novel therapeutics for treating autoimmune and allergic diseases.
Project description:Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development. Four condition experiment, stage I - IV individuals: 10 biological replicates per stage. Stages collected independantly from each other; sampling conducted over 2 seasons. Reference sample prepared from pooled RNA of 4 individuals per stage.
