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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

ABSTRACT: Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

PROVIDER: PRJEB39815 | ENA |

REPOSITORIES: ENA

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Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide chromatin accessibility patterns in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed ATAC-seq on a genome-wide scale. Combined with previous RRBS, RRHP and RNA-seq, the connections between DNA (hydroxyl)methylation in retroelements and gene expression were studied, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide DNA methylation and hydroxymethylation patterns as well as the transcription profiles in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed RNA-seq, reduced representation bisulfite sequencing (RRBS) and hydroxymethylation profiling (RRHP) on a genome-wide scale. We identified a large number of differential expressed genes (DEGs) and differential 5(h)mC signals across the whole genome. We discovered novel connections between DNA (hydroxyl)methylated modification and gene expression, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

Project description:Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

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Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

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Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Project description:Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goats to determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty.

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ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

Project description:We performed ATAC-seq on iPSC-derived hypothalamic arcuate-like neuron cells to identify putative regulatory elements. All samples were derived from the same individual and from the same differentiation/cell line but ATAC-seq was performed in 3 separate experiments (3 technical replicates).

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Transcription profiling of human hypothalamic hamartomas (HH) obtained from patients with or without precocious puberty (CPP)

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Gene expression data from hypothalamic hamartomas (HH) obtained from patients with or without precocious puberty (CPP)

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Project description:Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

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