Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

ABSTRACT: A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

PROVIDER: PRJEB40214 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus flower petals were infiltrated with Agrobacterium tumefaciens C58C1 or infiltration buffer as control. For each sample, flower petals from four to five flowers, each from a different individual plant were infiltrated.

2020-09-07 | E-MTAB-9517 | biostudies-arrayexpress

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus stems were dissected to separate the epidermis from the lower tissues. Leaves were dissected to obtain veins and veinless leaves. As controls, undissected leaves and stems were used. Three biological replicates were analyzed per sample.

2020-09-07 | E-MTAB-9516 | biostudies-arrayexpress

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus.

Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus hairy roots overexpressing the well-known MIA biosynthesis regulator ORCA3 were analyzed by RNA-Seq. As control, C. roseus hairy roots expressing GUS were used. Each analyzed sample consisted of an independent hairy root line; three hairy root lines per construct were analyzed.

2020-09-07 | E-MTAB-9514 | biostudies-arrayexpress

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus.

Project description:A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus.

| PRJEB40213 | ENA

A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

Project description:A modular system regulates specialized metabolite pathway branch choice in the medicinal plant Catharanthus roseus

| PRJEB40216 | ENA

Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus

Project description:The goal of this study is to investigate differential transcription profiles of leaf material/cells accumulating different levels of alkaloids in the anticancer plant Catharanthus roseus.

2023-10-16 | GSE217852 | GEO

Catharanthus Transcriptome and Gene Expression

2014-07-29 | GSE57326 | GEO

Catharanthus Transcriptome and Gene Expression

Project description:In this study, we aim to present a global transcriptome analysis of medicinal plant, Catharanthus roseus. We generated about 343 million high-quality reads from three tissues (leaf, root and flower) using Illumina platform. We performed an optimized de novo assembly of the reads and estimated transcript abundance in different tissue samples. The transcriptome dynamics was studied by differential gene expression analyses among tissue samples. We collected different tissue samples from the mature plants. Total RNA isolated from these tissue samples was subjected to Illumina sequencing. The sequence data was further filtered using NGS QC Toolkit to obtain high-quality reads. The filtered reads were used for de novo assembly optimization. The reads were further mapped to the Catharanthus transcripts via CLC Genomics Workbench and differential gene expression analysis was performed using DESeq software.

2014-07-29 | E-GEOD-57326 | biostudies-arrayexpress

Identification of New Branch Points and Unconventional Introns in Saccharomyces cerevisiae

Project description:Spliced messages constitute one-fourth of expressed mRNAs in the yeast Saccharomyces cerevisiae, and most mRNAs in metazoans. Splicing requires 5' splice site (5'SS), branch point (BP), and 3' splice site (3'SS) elements, but the role of the BP in splicing control is poorly understood because BP identification remains difficult. We developed a high-throughput method, Branch-seq, to map BP and 5'SS of isolated RNA lariats. Applied to S. cerevisiae, Branch-seq detected 76% of expressed, annotated BPs and identified a comparable number of novel BPs. We used RNA-seq to confirm associated 3'SS locations, identifying some 200 novel splice junctions, including an AT-AC intron. We show that several yeast introns use two or even three different BPs, with effects on 3'SS choice, protein coding potential, or RNA stability and identify novel introns whose splicing changes during meiosis or in response to stress. Together, these findings reveal BP-based regulation and demonstrate unanticipated complexity of splicing in yeast. 1 Lariat-seq experiment library. 3 barcoded Branch-seq libraries that make up one experiment. 26 RNA-seq samples, 2 biological replicates of each.

2016-08-01 | E-GEOD-68022 | biostudies-arrayexpress

Catharanthus longifolius

Project description:Catharanthus longifolius overview

| PRJNA178690 | ENA