Project description:Gene expression profiles of individual bone marrow cells were acquired by Drop-Seq. Subsets of bone marrow cells were isolated using fluorescence-assisted cell sorting to enrich for putative skeletal stem cells.
Project description:RATIONALE: Radiation therapy uses high-energy x-rays to damage cancer cells. Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells.
PURPOSE: Phase II trial to study the effectiveness of bone marrow transplantation in treating patients who have hematologic cancer.
Project description:Gene expression profiles of individual bone marrow cells were acquired by Drop-Seq. Subsets of bone marrow cells were isolated using magnetic cell sorting to enrich for putative skeletal stem cells.
Project description:We analyzed bone marrow aspirates from seven patients with vivax malaria and RNAseq analysis identified genes assoviated with erythropoietic defects
Project description:Human bone marrow mesenchymal stem cells (MSCs) were co-cultured for 7 days with endothelial cells, where they participated in the formation of microcapillaries. MSCs that were exposed to the microcapillaries or kept as monocultures were isolated by FACS and analyzed by RNAseq.