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MicroRNA expression profile in Newcastle disease virus-infected HeLa cells

ABSTRACT: MicroRNA expression profile in Newcastle disease virus-infected HeLa cells

PROVIDER: PRJEB40897 | ENA |

REPOSITORIES: ENA

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MicroRNA expression profile in Newcastle disease virus-infected HeLa cells

Project description:Three small RNA libraries constructed from uninfected HeLa cells or the cells infected with NDV strain Herts/33 at 6 and 12 hr post inoculation were sequenced using an Illumina platform.Three replicates of each group (Mock, NDV-6h and NDV-12h) were prepared and pooled separately for library construction and small RNA Deep sequencing.

2020-11-08 | E-MTAB-9690 | biostudies-arrayexpress

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Transposon mutagenesis screening of Newcastle disease virus (NDV)

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RNA-Seq Analysis of Spleen from Chicken and Japanese Quail infected with Lentogenic and Velogenic Newcastle Disease Virus

Project description:Newcastle disease (ND) affects a few hundred avian species including chicken, and the clinical outcome of Newcastle disease virus (NDV) infection ranges from mild to severe fatal disease depending on the NDV pathotype and the host species involved. Japanese quails serve as natural reservoirs of NDV and play important role in NDV epidemiology. While infection of chicken with velogenic NDV results in severe often fatal illness, the same infection in Japanese quails is results in in apparent infection. The molecular basis of this contrasting clinical outcomes of NDV infection is not yet known. We compared global gene expression in spleens of chicken and Japanese quails infected with a lentogenic or velogenic NDVs. We found contrasting regulation of key genes associated with NF-κB pathway and T-cell activation between chicken and Japanese quails. Our data suggests association of NDV resistance in Japanese quails to activation of NF-κB pathway and T cell proliferation.

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Project description: Not available

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Transcriptome profiling data of urothelial cell carcinoma (UCC) persistently infected with oncolytic Newcastle disease virus

Project description:Newcastle disease virus (NDV) is an avian virus that selectively replicates and kills many different types of cancer cells and is being developed for cancer treatment. Our aim was to establish persistent infection in EJ28 and TCCSUP bladder cancer cells and identify the dysregulated genes and disrupted molecular pathways associated with persistent infection.

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Expression data of spleens from chickens infected with different Newcastle Disease Viruses

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Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

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Expression data of spleens from chickens infected with different Newcastle Disease Viruses

Project description:Genotype VIId NDV is characterized by severe tissue damage in chicken lymphoid organs compared to other virulent strains. However, biological basis of this unusual pathological phenotype is unknown. Host response is associated with pathogenicity of Newcastle Disease Virus (NDV). We aim to determine the contribution of host response to the severe tissue destruction in the lymphoid system caused by genotype VIId NDV. We used microarray analysis to evaluate the global transcriptional response in the spleen of chickens infected with genotype VIId NDV strain JS5/05 and genotype IV NDV Herts/33. Chickens were inoculated with JS5/05 or Herts/33 or mock-infected. At day 2 post infection, spleens were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: JS5/05 (I4_1_NS,I4_2_NS,I4_3_NS), Herts/33 (H1_NS,H2_NS, H3_NS), control (C1_NS, C2_NS, C3_NS).

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