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RNA-seq of small RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence

ABSTRACT: RNA-seq of small RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence

PROVIDER: PRJEB41024 | ENA |

REPOSITORIES: ENA

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RNA-seq of small RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence

Project description:The intent of the experiment was to identify differentially expressed micro-RNAs comparing WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

2020-11-02 | E-MTAB-9710 | biostudies-arrayexpress

ChIP-seq of H3-K4me3 from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:The intent of the experiment was to compare H3-K4me3 peaks on the chromatin of WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

2020-11-02 | E-MTAB-9718 | biostudies-arrayexpress

RNA-seq of rRNA-depleted total RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:The intent of the experiment was to identify differentially expressed RNAs comparing WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

2020-11-02 | E-MTAB-9714 | biostudies-arrayexpress

ChIP-seq of RNA polymerase II from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:The intent of the experiment was to compare RNA polymerase II occupancy on the chromatin of WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

2020-11-02 | E-MTAB-9717 | biostudies-arrayexpress

RNA-seq of sh2-H2AFJ and sh-NoTarget in WI-38hTERT cells induced into senescence by etoposide

Project description:Study the role of H2A.J in gene expression in senescent human fibroblasts by comparing RNA-seq of WI-38hTERT fibroblasts expressing either an sh2-H2AFJ or sh-NoTarget RNAs. Senescence was induced by treating cells with 20 M etoposide for 2 weeks followed by one further week of incubation without etoposide. 3 biological replicates of sh2-H2AFJ and sh-NoTarget were sequenced.

2016-07-21 | E-MTAB-4920 | biostudies-arrayexpress

ChIP-seq of H3-K4me3 from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:ChIP-seq of H3-K4me3 from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

| PRJEB41065 | ENA

ChIP-seq of RNA polymerase II from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:ChIP-seq of RNA polymerase II from WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

| PRJEB41064 | ENA

RNA-seq of rRNA-depleted total RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

Project description:RNA-seq of rRNA-depleted total RNAs in WI-38hTERT/sh3-H2AFJ and WI-38hTERT/sh-NT fibroblasts in proliferation and etoposide-induced senescence.

| PRJEB41051 | ENA

RNA-seq of sh2-H2AFJ and sh-NoTarget in WI-38hTERT cells induced into senescence by etoposide

Project description:RNA-seq of sh2-H2AFJ and sh-NoTarget in WI-38hTERT cells induced into senescence by etoposide

| PRJEB14765 | ENA

Transcriptome analysis by Illumina microarrays of the effect of overexpressing H2A.J mutants, or WT H2A.J or canonical H2A, on the transcriptome of WI-38hTERT fibroblasts

Project description:The intent of the experiment was to test the effect of overexpressing H2A.J-V11A, H2AJ-S123E, H2A.J-S123A, and H2A.J-CterH2A mutants, as well as WT-H2A.J and canonical H2A-type1, on the transcriptome of WI-38hTERT fibroblasts. pTRIPz based lentiviral constructs placing these coding sequences under tetON control were used to produce stable WI38hTERT cell lines for each construct. Expression of the constructs in proliferating cells was induced with 100 ng/ml doxycycline for è days. Transcriptomes were also compared with control proliferating WI-38hTERT and WI-38hTERT cells induced into senescence by incubation with 20 µM etoposide for 2 weeks followed by 1 week without etoposide.

2020-11-01 | E-MTAB-9706 | biostudies-arrayexpress

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