Project description:four biological replicates of arabidopsis accession columbia 78h old whole seedling expression data Keywords: exon/intergenic comparison

2006-12-19 | GSE6562 | GEO

Peptide sequences generated by unexpected splicing

Project description:Transcripts from the human and mouse CAPN10 cDNA sequences cloned into pcDNA and expressed in cultured cells undergo unexpected splicing. Peptide sequences missing from the spliced version of CAPN10 expressed in COS7 cells were determined by MS analysis.

2022-01-01 | PXD026551 | JPOST Repository

Analysis of a normalised equine cDNA library using deep sequencing

Project description:While a first draft of the equine genome is available and predictions are made regarding resulting genes and proteins, little is known about the actual transcriptome. So far, published expressed sequence tags (ESTs) from different horse tissues were generally rather short (≤600bp) and hardly annotated, reflecting the problem that good cDNA libraries are very difficult to analyse. In this approach, we aimed to establish and analyse a normalised immune cell cDNA library (using freshly isolated and activated lymphocytes, NK cells, monocytes and DC). In particular, we wanted to test next generation sequencing combined with a series of bioinformatic approaches. The resulting cDNA library contained 2x107 clones of which 1056 were used for an initial Sanger sequencing and 4x106 for the deep sequencing analysis. Through the latter we obtained >29k sequences for which more than 5000 matches where found on the equine reference sequences. Additionally we could identify more than 3500 sequences which had matches on both - non-equine RNA sequences as well as the equine genome. In these we find both extensions of existing RefSeq models and novel mRNAs alike. Less than 2% of sequences did not have any match in the mentioned databases.

2012-06-30 | GSE26794 | GEO

blueberry transcriptome cDNA sequencing

Project description:blueberry transcriptome cDNA sequencing

| PRJEB23599 | ENA

seawater cDNA samples

Project description:seawater cDNA samples Raw sequence reads

| PRJNA497918 | ENA