Project description:In this study, we infected rat with the intestinal protist Blastocystis ST3 and measure the impact on the gut microbiota. To characterised the gut microbiota, we amplified the V4 region of the 16s rRNA gene according to the EMP protocol and sequence were amplified using Illumina Miseq.
Project description:Mouse infection with the tapeworm Hymenolepis diminuta leads to a less severe DNBS-colitis. Increased Th2 and regulatory cytokine production in the spleen is a hallmark of Hymenolepis diminuta infection, therefore we hypothesized that given this microenvironment, splenic adaptive cells acquire an anti-inflammatory phenotype. We tested the ability of putative splenic regulatory B cells generated by Hymenolepis diminuta infection to down-regulate intestinal inflammation. We found that unlike splenic B cells from uninfected mice, splenic B cells from Hymenolepis diminuta -infected animals ameliorated chemically-induced colitis. Splenic B cells were magnetically isolated and also purified by cell sorting from 7 days-infected animals and B cells from uninfected animals were used as control.
Project description:Mouse infection with the tapeworm Hymenolepis diminuta leads to a less severe DNBS-colitis. Increased Th2 and regulatory cytokine production in the spleen is a hallmark of Hymenolepis diminuta infection, therefore we hypothesized that given this microenvironment, splenic adaptive cells acquire an anti-inflammatory phenotype. We tested the ability of putative splenic regulatory B cells generated by Hymenolepis diminuta infection to down-regulate intestinal inflammation. We found that unlike splenic B cells from uninfected mice, splenic B cells from Hymenolepis diminuta -infected animals ameliorated chemically-induced colitis.
Project description:Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel.
Project description:Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the ileal gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel.
