Project description:Comparative genomic hybridization of 9 Norwegian E. faecalis baby isolates with E. faecalis V583 as a reference strain using an E. faecalis V583 oligo array. Total gene content was analyzed by whole genome microarrays.
Project description:The ongoing COVID-19 pandemic caused by SARS-CoV-2 has affected millions of people worldwide and has significant implications for public health. Host transcriptomics profiling provides comprehensive understanding of how the virus interacts with host cells and how the host responds to the virus. COVID-19 disease alters the host transcriptome, affecting cellular pathways and key molecular functions. To contribute to the global effort to understand the virus’s effect on host cell transcriptome, we have generated a dataset from nasopharyngeal swabs of 35 individuals infected with SARS-CoV-2 from the Campania region in Italy during the three outbreaks, with different clinical conditions. This dataset will help to elucidate the complex interactions among genes and can be useful in the development of effective therapeutic pathways
Project description:Effects of heifer feeding regimes on growth characteristics, metabolism, endocrinology, health and subsequent milk yield has been described in several studies. This indicates that nutritional impacts have both short- and long-term effects on the animal’s metabolic regulation. Adipose tissue has an important role in whole-body metabolic regulation, as it is the main site for both lipid synhtesis, storage and mobilization as well as an endocrinologically active organ. However, little is known about bovine adipose metabolism at gene level, and what is done is mostly found in mature cows by means of microarrays. The goal of this study was to apply next-generation RNA sequencing on the adipose tissue of Norwegian Red heifers fed differently, in order to gain knowledge about nutritionally induced regulatory mechanisms in growing dairy cattle. The objectives were to: 1) Describe the differential gene expression in the adipose tissue of heifers fed diets differing in energy and protein content; 2) Describe the long-term changes in adipose gene expression occuring within treatment groups between the experimental feeding period and 6 months after the transition from experimental diets to a similar diet offered to all animals; 3) Identify any post-treatment effects of the experimental feeding as expressed by differential gene expression between treatment groups still present 6 months after the termination of experimental feeding; and 4) To identify candidate genes for further studies on the breeding and feeding for improved feed efficiency and production in the Norwegian Red.
Project description:The urgent approval of the use of the inactivated COVID-19 vaccine is essential to reduce the threat and burden of the epidemic on global public health, however, our current understanding of the host immune response to inactivated vaccine remains limited. We performed transcriptomics analysis on 20 SARS-CoV-2 naïve individuals who received multiple doses of inactivated vaccine and five SARS-CoV-2 recovered individuals who received single dose of inactivated vaccine. These data help us understand the reaction mechanism of the host's molecular immune system to the inactivated vaccine, and provide a basis for the choice of vaccination strategy.
