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3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

ABSTRACT: 3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

PROVIDER: PRJEB43428 | ENA |

REPOSITORIES: ENA

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3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

Project description:To study the involvement of key Areg (CD142+ ASPC)-specifics factors in the inhibitory capacity of CD142+ ASPCs on adipogenesis, we performed transcriptomic profiling of CD142− ASPCs exposed to the secretome of CD142+ ASPCs carrying knockdowns of the indicated genes. CD142− ASPCs were co-cultured and co-differentiated (within the transwell set-up) with CD142+ ASPCs in which siRNA-based knockdowns of specific genes were performed. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

2022-07-04 | E-MTAB-10183 | biostudies-arrayexpress

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Project description:To validate that the robustness of Aregs' (CD142+ ASPCs') molecular identity regardless of the antibody used, we performed transcriptomic profiling of freshly isolated CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs) sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ CD142+/− cells of the mouse subcutaneous stromal vascular fraction using FACS.

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3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies

Project description:3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies

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3’RNA-seq of murine fresh and expanded ASPCs

Project description:To characterize murine ASPCs we performed bulk RNA-seq (BRB-seq, Alpern et al., 2019) of total, CD142− and CD142+ (Aregs) mouse adipose stem and progenitor cells (ASPCs). ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS. The cells were sequenced directly after sort or after expansion.

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3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

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Project description:To validate the anti-adipogenic effect of the newly discovered Areg cells in human, we used FACS to separate the CD142+ from the CD142- population within cultured (passage 1) adipogenic stem and progenitor cells (ASPCs) (CD31- CD45- stromal vascular fraction cells) that were derived from four individuals, and induced adipogenesis. We then performed 3 RNA-seq expression profiling of the induced cells.

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