Project description:Cryptococcal osteomyelitis is an infrequent infection which is usually associated with disseminated cryptococcosis or underlying immunocompromised conditions. Here we described a rare case with isolated iliac cryptococcosis in an immunocompetent patient. Through histological, microbial, and molecular biological examinations, the pathogen was finally identified as C. neoformans VNI genotype, which likely originated from environmental bird droppings. The clinical isolate was hypomelanized but fully virulent in mouse infection model. The patient displayed lower CD4+-T lymphocyte ratio, reduced serum IFN-γ and IL-12, and dysregulated transcriptional profile of blood leukocytes compare with healthy host. After surgical excision and 34 weeks’ antifungal treatment, the patient got clinical cured. Our study suggested that cryptococcosis development was closely associated with the interaction of fungal agent and host immunity. Accurate diagnosis of bone cryptococcosis depends mainly on histological and fungal examinations. A combination of antifungal agent treatment regimen and surgery were quite effective for resolving bone cryptococcosis.

2018-04-17 | GSE108534 | GEO

Metagenomic Next-generation Sequencing assists in the diagnosis of Cryptococcus pneumoniae

Project description:Metagenomic Next-generation Sequencing assists in the diagnosis of Cryptococcus pneumoniae

| PRJEB43820 | ENA

Invasive Pulmonary Aspergillosis Diagnosis via Peripheral Blood Metagenomic Next Generation Sequencing

Project description:Invasive Pulmonary Aspergillosis Diagnosis via Peripheral Blood Metagenomic Next Generation Sequencing

| PRJEB43573 | ENA

Next-generation diagnosis of bacteremia in septic patients

Project description:Next-generation diagnosis of bacteremia in septic patients

| PRJEB13247 | ENA

Metagenomic next-generation sequencing for mixed pulmonary infection diagnosis.

Project description: Not available