Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

ABSTRACT: Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

PROVIDER: PRJEB43593 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Innate lymphoid cells

Project description:Innate lymphoid cells (ILC) in the small intestine govern immune homeostasis and protect the host against gut pathogens. While distinct cell-intrinsic signals have been identified that determine ILC development and differentiation, it has remained unclear which cell population regulates ILC sustenance. Using unbiased single cell RNA transcriptomic analysis of intestinal fibroblasts, we have identified a specialized Ccl19-expressing fibroblastic reticular cell (FRC) population that underpins solitary intestinal lymphoid tissue (SILT) structures including cryptopatches and isolated lymphoid follicles. Conditional ablation of lymphotoxin-β receptor (LTβR) signalling in SILT FRC impeded the maturation of isolated lymphoid follicles and blocked ILC maintenance through the downregulation of IL-7, consequently resulting in the elevated susceptibility to bacterial infection. Moreover, specific Ltbr ablation in FRC during adulthood revealed that constant LTβR-dependent FRC-ILC interaction is required to maintain SILT structures and ILC populations. Taken together, our study unveils a critical intestinal FRC niche that secures protective gut immunity.

2022-02-12 | E-MTAB-10645 | biostudies-arrayexpress

Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

Project description:In an effort to define transcriptional heterogeneity in the stromal cell compartment of lymph nodes scRNA-seq analysis of CD45- cells from skin-draining LNs (SLN) was performed. Flow sorted CD45 depleted single cell suspension from SLN of ten mice were profiled by 10x 3’ single-cell RNA-sequencing in two runs, each containing cells from five mice.

2021-04-10 | E-MTAB-10197 | biostudies-arrayexpress

Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Intestinal fibroblasts

2022-02-12 | E-MTAB-10638 | biostudies-arrayexpress

Gremlin 1 + fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

Project description:Although cross-species transcriptional analysis has been generated for DCs37, transcriptomic conservation between mouse and human FRCs at single-cell resolution has been unclear. To test whether GREM1+ FRCs might also play a role in DC homeostasis in humans, we performed scRNA-seq of CD45Ã¢Â€Â“PDPN+ stromal cells, as well as CD45+CD11c+ immune cells from healthy human LNs of three donors.

| EGAS00001006211 | EGA

Single-cell RNA sequencing reveals tissue-associated fibroblastic reticular cell heterogeneity in human secondary lymphoid organs

Project description:Fibroblastic reticular cells (FRCs) actively support secondary lymphoid organ (SLO) architecture. They regulate innate and adaptive immunity by compartmentalizing immune cells in specialized niches, and tightly interacting with them. The phenotypic and functional diversity of FRCs in human SLOs remains largely uncharacterized. We performed a comprehensive profiling of FRCs by single-cell RNA sequencing on human tonsils and lymph nodes. We provide the first complete description of the tonsillar CD45-negative compartment. We identified and validated in tonsil, a population of follicular dendritic cells restricted to the mantle zone and discovered two CCL21-negative FRC subsets intermingling with T-cell zone reticular cells in the extrafollicular space. We also identified podoplanin-positive epithelial cells as a potential plasma cell (PC) niche. We found two levels of FRC-associated diversity: i) tissue-specific subsets; ii) shared subsets with tissue-specific gene signatures. These findings shed new light on the role of FRCs in supporting SLO-specific immune responses.

2023-02-01 | GSE193449 | GEO

Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Innate lymphoid cells

Project description:Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Innate lymphoid cells

| PRJEB46051 | ENA

Activation of TLR9 signaling suppresses the immuno modulating functions of CD55lo fibroblastic reticular cells during bacterial peritonitis

Project description:Fibroblastic reticular cells (FRCs) are heterogeneous. We use single cell RNA sequencing to identify subsets of FRCs in fat-associated lymphoid clusters (FALC) in the mesenteric adipose tissue

2024-04-18 | GSE250571 | GEO

Snai2 maintains bone marrow niche cells by repressing osteopontin expression

Project description:Snai2 maintains bone marrow niche cells by repressing osteopontin expression

| PRJNA598042 | ENA

Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Intestinal fibroblasts

Project description:Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function - Intestinal fibroblasts

| PRJEB46101 | ENA

Hedgehog gradient in the stroma maintains niche diversity and organ function

Project description:Hedgehog gradient in the stroma maintains niche diversity and organ function

| PRJNA398101 | ENA