Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of early- and late-passage human mesenchymal stem cells (5 donors, donor-matched) with and without treatment with 2 hours of 42 degrees C heat shock

ABSTRACT: RNA-seq of early- and late-passage human mesenchymal stem cells (5 donors, donor-matched) with and without treatment with 2 hours of 42 degrees C heat shock

PROVIDER: PRJEB44754 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of early- and late-passage human mesenchymal stem cells (5 donors, donor-matched) with and without treatment with 2 hours of 42 degrees C heat shock

Project description:Understanding how the response of human cells respond to protein-unfolding stress changes with cellular age is important to gain a better understanding of the link between ageing and protein-folding diseases. The experiment was designed to assess the total mRNA levels in heat shocked cells from proliferating (early-passage) and senescent (late-passage) cultures; and was used, in conjunction with mass spectrometry data, to gain an insight into the systemic response to protein-unfolding stress. Here, total RNA was extracted from early- and late-passage human mesenchymal stem cells (n=5, donor matched) following 2 hours of heat shock at 42 degrees C. RNA from cells kept at 37 degrees C was used as control. The protein coding RNAs were then sequenced with Illumina HiSeq technology.

2022-04-18 | E-MTAB-10415 | biostudies-arrayexpress

Global gene expression analysis of donor cells, low passage episomal stromal-primed myeloid iPSC, and intermediate passage fibroblast iPSC.

Project description:Global gene expression analysis of (a) human embryonic stem cells, (b) low passage episomal stromal-primed myeloid iPSC, (c) intermediate passage fibroblast iPSC, and (d) samples of corresponding donor cells.

2016-09-13 | GSE44421 | GEO

Translational profiling of HEK293 cells exposed to cold shock (32 degrees C) and untreated (37 degrees C)

Project description:This experiment investigates the effect of mild cold-shock (32 degrees C, 24 hours) on the translatome of human HEK293 cells. After cold-shock, cells were treated with cycloheximide to block elongation, then sucrose density gradients were used to separate post-nuclear extracts into polysome and subpolysome fractions. The pooled polysomal fraction was labelled with Cy5, the pooled subpolysomal fraction was labelled with Cy3, and both were hybridised to the same array.

2017-02-23 | E-MTAB-5437 | biostudies-arrayexpress

Simulation shows that HLA-matched stem cell donors can remain unidentified in donor searches.

Project description: Not available

| S-EPMC4753406 | biostudies-literature

The comparison of gene expression profiles in hepatic stellate cells isolated from mouse livers by perfusion method at 37 degrees or 6 degrees

Project description:We compared transcriptome profiling (bulk RNA-seq) of hepatic stellate cells isolated from mouse livers by perfusion method using collagenase at 37 degrees or 6 degrees and evaluated the heat-stressed gene expression at 37 degrees.

2022-09-28 | GSE192598 | GEO

Transcription profiling of human HUVEC (Human Umbilical Vein Endothelial Cells) cells exposed to heat shock (42 degrees Celcius) for different time points (1hr, 3 hrs, 6 hr and 12 hrs) and vs non heat-shock cells with varying amounts of external spikes added

Project description:HUVEC (Human Umbilical Vein Endothelial Cells) cells were exposed to heat shock (42 degrees Celcius) for different time points (1hr, 3 hrs, 6 hr and 12 hrs) and compared to non heat-shock cells. External normalization controls were added in equal amounts to equivalent amounts of total RNA.

2003-04-01 | E-UMCU-2 | biostudies-arrayexpress

The expression profiles of log-phase and stationary-phase cells of Shigella flexneri grown at 30 degrees and 37 degrees Celsius

Project description:To obtain a global view of the response of S. flexneri at the expression level induced by temperature up-shift, we compared the expression profiles of log-phase and stationary-phase cells grown at 30 degrees and 37 degrees Celsius.

2010-06-02 | GSE16535 | GEO

Global gene expression analysis of donor cells, low passage episomal stromal and non-stromal primed myeloid iPSC, and hESC

Project description:Global gene expression analysis of (a) human embryonic stem cells, (b) low passage episomal stromal-primed iPSC derived from growth-factor activated CD34+ myeloid progenitors from cord blood or GCSF mobilized peripheral blood, and (d) samples of donor fibroblasts or myeloid progenitors

2016-09-13 | GSE44427 | GEO

Gene expression profile of abf1-1 mutant at 36 degrees C

Project description:The abf1-1 mutant and wild type cells were grown at 30 degrees C in YPAD until OD600 reached 1.0-1.2. The same volume of 42 degrees C preheated YPAD was added for each flask. After 45 min incubation at 36 degrees C, cells were harvested and RNA was isolated. Four repeats of microarray results are in this series. Keywords: repeat sample

2004-06-23 | GSE1492 | GEO

Early and late passage endothelial cells with or without PDCD10 downregulation

Project description:Primary cells deficient for PDCD10/CCM3 do not enter senescence as control cells. Microarray analysis was performed in cells transduced with non-targeting shRNA and CCM3 shRNA at passage 7 (early passage) and passage 11 (late passage), when control cells are already senescent.

2014-04-10 | GSE54095 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data