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Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

ABSTRACT: Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

PROVIDER: PRJEB44757 | ENA |

REPOSITORIES: ENA

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Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

Project description:Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is the workhorse of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proliferate in suspension to high cell numbers. The molecular mechanisms that facilitate the adaptation and that take place inside the cell are largely unknown. Especially for cell lines that are used for virus antigen production such as baby hamster kidney (BHK) cells, the restriction of virus growth through the evolution of undesired cell characteristics properties is highly unwanted. The comparison between adherently growing BHK cells and suspension cells with different susceptibility to foot-and-mouth disease virus revealed differences in the expression of cellular receptors such as integrins and heparan sulfates, and in the organization of the actin cytoskeleton. Transcriptome analyses and growth kinetics demonstrated the diversity of BHK cell clones and confirmed the importance of well-characterized parental cell clones and mindful screening to make sure that essential cellular features do not get lost during adaptation.

2021-05-15 | E-MTAB-10417 | biostudies-arrayexpress

Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.

Project description: Not available

| S-EPMC8177424 | biostudies-literature

Expression analysis of doxycycline inducible secondary fibroblasts reprogramming under adherent and suspension conditions

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Expression analysis of doxycycline inducible secondary fibroblasts reprogramming under adherent and suspension conditions

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Identifying differences in differentiation between adherent and suspension culture for iPSC derived beta cells

Project description:Stage 3 cells from a suspension environment and adherent culture were compared and evaluated through RNA sequencing. RNA sequencing data in heatmap and volcano plot evaluation of core endocrine genes showed that expression levels in adherent cells were lower than those expressed in 3D environment. Several genes that are essential for insulin transcription such as NEUROD1, MAFA, PPARGC1A and NKX2.2 along with INS were expressed with higher transcript levels in bioreactor samples. It was observed that genes usually expressed in earlier stages such as FOXA2 and HNF1b had higher expression levels in adherent culture indicating that 2D environment differentiation might be lagging.

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RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

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Transcriptomic Analysis on Oncogene STYK1/NOK transfected Baby Hamster Kidney BHK21 Cells

Project description:Transcriptomic Analysis on Oncogene STYK1/NOK transfected Baby Hamster Kidney BHK21 Cells

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Differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell lines CAL27 and HSC3

Project description:To investigate the differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell, We compared the difference in gene expression between CAL27 and HSC3 cultured in suspension culture and adherent culture.

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RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

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Gene expression profiles during the establishment of CHK-Q (Chinese hamster kidney-derived cell line) and derivative cells

Project description:CHK-Q cell line was established from primary culture of Chinese hamster kidney tissues by repeated passages. DNA microarray analysis for chasing the variation in gene expression profile was performed for CHK cells during the culture process leading to immortalization and adaptation to serum-free and suspension conditions.

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