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Anopheles funestus FANG vs FUMOZ RNASeq

ABSTRACT: An RNAseq-based gene expression profiling of the Anopheles funestus pyrethroid resistant strain FUMOZ highlights the predominant role of the duplicated CYP6P9a/b cytochrome P450s.

PROVIDER: PRJEB45224 | ENA |

REPOSITORIES: ENA

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Anopheles funestus strain:FUMOZ

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Project description:Transcriptome profiling of pyrethroid resistant field populations of Anopheles funestus from Malawi and Mozambique compared to a susceptible lab strain FANG

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De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology

Project description:We report the use of RNA-seq data to assemble transcriptional units of adult Anopheles funestus female mosquitoes. We also analyzed expression levels and protein divergence and discovered SNPs.

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Transcription profiling of bendiocarb resistance in Anopheles funestus

Project description:Transcription profiling of bendiocarb resistance in Anopheles funestus

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Anopheles funestus amidst a South/North shift in the role of key resistance genes across Malawi

Project description:Resistance to pyrethroids, the only insecticide approved for bednets, threatens control of the major malaria vector, Anopheles funestus, in Malawi. To improve the management of such resistance countrywide, it is crucial to understand the dynamics and mechanisms driving resistance in the field. In this study the levels of insecticide resistance were determined across the highly endemic densely populated lake and southern agricultural area. Insecticide resistance to pyrethoids was assessed using standardized WHO bioassay methods and resistant mosquitoes were hybridized to susceptible mosquitoes. This microarray analysis revealed the key role of cytochrome P450 genes such as CYP6P9a, CYP6P9b and CYP6M7. However, a significant shift in the over-expression of these CYP450s was detected across a south/north transect, with CYP6M7 more highly over-transcribed in the two northern collection sites and the tandemly duplicated genes, CYP6P9a and CYP6P9b, more greatly over-transcribed in the south.

2016-02-12 | E-MTAB-3342 | biostudies-arrayexpress

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