Project description:4T1 mouse tumor cells were screened with a CRISPR library concurrently in vivo in both SCID and immunocompetent mice. Screens were also carried out in vitro.
Project description:Genome-wide CRISPR-Cas9 knockout screen using TKOv1 sgRNA library was performed in isogenic RBM10-proficient and RBM10-deficient HCC827 cells.
Project description:We present a CRISPR-based multi-gene knockout screening system and new toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations, and successfully validated double and triple combination regimens for suppression on cancer cell growth and protection against Parkinson’s disease-associated toxicity. This system overcomes the practical challenges to experiment on a large number of high-order genetic and drug combinations, and is applicable for uncovering the rare synergistic interactions between druggable targets.
Project description:C57BL/6 mouse pancreas cells were infected with a CRISPR library targeting TSGs. Total HLA and SIINFEKL presentation was measured by flow and cells were sorted for sequencing