Project description:Intent: to investigate whether treatment of an individual with peptide immunotherapy using MultiPepT1De results in a change in the transcriptional profile of CD4+ regulatory T cells. Regulatory T cells (CD4+ CD25+ CD127lo) were isolated by flow cytometry cell sorting from cryopreserved PBMC from subjects before and after treatment with MultiPepT1De. RNA was extracted using the AllPrep micro kit (Qiagen) and RNAseq libraries prepared from extracted RNA (91-140 ng)
Project description:RNAseq analysis of human CAR-Tregs, bearing CD28 or 4-1BB as a costimulatory domains, initially isolated (before engineering) from peripheral blood of healthy donors
Project description:RNAseq of wild type Col-0 Arabidopsis plants and piezo/feronia/camta3 mutants before and 22 minutes after touch treatment with a gentle paint brush. The mutants were defective in genes involved in mechanosensing and downstream signalling. Their contribution to touch-responsive signalling was assessed by transcriptome analysis.
Project description:The aim of the project is to do proteomic analysis of total cell lysate from rest and activated mouse Tregs. The proteomic data were mapped to the Gene Ontology Cellular Component (GOCC)database to obtain the list of membrane proteins on activated Tregs. The set of membrane proteins identified were compared with the potential Siglec-1 counter-receptors on activated Tregs.
Project description:Proteins immunoprecipitated by Neil3 in 4 conditions
- before Cisplatin treatment with inhibition of proteasome
- before Cisplatin treatment without inhibition of proteasome
- after 3h of Cisplatin treatment with inhibition of proteasome
- after 3h of Cisplatin treatment without inhibition of proteasome