Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Viruses of methanogenic archaea in anaerobic digestion reactors

ABSTRACT: In situ characterization of the genomic content of viruses of methanogenic archaea in anaerobic digestion reactors

PROVIDER: PRJEB46489 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genomic hybridization of Methanobacteria to identify the diversity in strains on manure and olive mill waste.

Project description:Population dynamics of methanogenic genera was investigated in pilot anaerobic digesters. Cattle manure and two-phase olive mill wastes were codigested at a 3:1 ratio in two reactors operated at 37 ï¾°C and 55 ï¾°C. Other two reactors were run with either residue at 37 ï¾°C. Sludge DNA extracted from samples taken from all four reactors on days 4, 14 and 28 of digestion was used for hybridisation with the AnaeroChip, an oligonucleotide microarray targeting those groups of methanogenic archaea that are commonly found under mesophilic and thermophilic conditions (Franke-Whittle et al. 2009, in press, doi:10.1016/j.mimet.2009.09.017).

2010-11-10 | E-MEXP-2453 | biostudies-arrayexpress

methanogenic archaea

Project description:methanogenic archaea Raw sequence reads

| PRJNA497484 | ENA

Dispensable Methanogenic Energy Metabolism in a Methanogenic Archaeon

Project description:Biogenic methane formation, methanogenesis, a key process in the global carbon cycle is the only energy metabolism known to sustain growth of the microorganisms employing it, the methanogenic archaea. All known methanogenic pathways converge at the methane-liberating step where also the terminal electron acceptor of methanogenic respiration, the heterodisulfide of coenzyme M and coenzyme B is formed. Carbon monoxide (CO) utilization of Methanosarcina acetivorans is unique in that the organism can shift from methanogenesis towards acetogenesis. Here, we show that M. acetivorans can dispense of methanogenesis for energy conservation completely. By disrupting the methanogenic pathway through targeted mutagenesis, followed by adaptive evolution, a strain capable of sustained growth by CO-dependent acetogenesis was created. Still, a minute flux through the methane-liberating reaction remained essential, which was attributed to the involvement of the heterodisulfide in at least one essential anabolic reaction. Genomic and proteomic analysis showed that substantial metabolic rewiring had occurred in the strain. Most notably, heterodisulfide reductase, the terminal respiratory oxidoreductase was eliminated to funnel the heterodisulfide towards anabolism. These results suggest that the metabolic flexibility of “methanogenic” archaea is much greater than anticipated and open avenues for probing the mechanism of energetic coupling and the crosstalk between catabolism and anabolism.

2021-10-05 | PXD022016 | Pride

MICROBIAL COMMUNITIES AT METHANOGENIC REACTORS

Project description:METAGENOMIC ANALYSIS OF HIGHLY ADAPTED MICROBIAL COMMUNITIES AT METHANOGENIC REACTORS IN SINGLE AND TWO-STAGE SYSTEMS TREATING RAW SUGARCANE STILLAGE

| PRJEB8616 | ENA

TMT proteomic analysis of Methanogenic archaeon

Project description:Proteomic quantitative analysis of the methanogenic archaea in the experimental group and the control group,each group was repeated three times.For each 6-plex TMT,control samples were labelled with TMT tags 126,127,128,and experimental samples were labelled with TMT tags 129,130,and 131,respectively

2020-07-15 | PXD020372 | iProX

Anaerobic digestion samples (C for cycles and R for reactors)

Project description:Anaerobic digestion samples (C for cycles and R for reactors) Raw sequence reads

| PRJNA824252 | ENA

methanogenic archeae

Project description:Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence.

| PRJNA379847 | ENA

Genomes of Choline-utilising Methanogenic Archaea, Methanococcoides spp. from Marine Sediments

Project description:Genome Sequences of Choline-utilising Methanogenic Archaea, Methanococcoides spp. Isolated from Marine Sediments

| PRJEB31721 | ENA

MMP103

Project description:Methanococcus maripaludis is a methanogenic Archaea that conserves energy from molecular hydrogen to reduce carbon dioxide to methane. Chemostat grown cultures limited for phosphate or leucine were compared to determine the regulatory response to leucine limitation. Keywords: archaea, hydrogen, leucine, phosphate, nutrient limitation, growth rate, methanogen

2007-08-30 | GSE8728 | GEO

caprylate reactors

Project description:caprylate reactors

| PRJEB21836 | ENA