Project description:ATAC-seq of Col and hcr2 mutant from seedling and bud sample. Tagmentation was performed using the Tag DNA enzyme & buffer kit (Illumina, 20034210). Nextera DNA CD Index primers were used for library construction. The indexed libraries were subjected to paired-end 50-bp sequencing using an Illumina HiSeqX instrument (Microgen, Korea).
Project description:RNA-prep of Col and hcr2 mutant from seedling and bud sample. RNA extracted by Trizol and cDNA was synthesized by using the ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre). ScriptSeq Index PCR Primers (RSBC10948, Epicentre) were used for library construction.
Project description:DNA-prep of Col and hcr2 mutant from seedling and bud sample. DNA was extracted using DNeasy Plant Mini Kit (Qiagen 69104, USA). Library construction and bisulfite conversion was performed using EZ DNA Methylation-Gold kit (ZYMO). DNA libraries are sequenced on the DNBseq platform (BGI, Hong Cong).
Project description:Arabidopsis thaliana Col-0 plants were compared to sir1-1 T-DNA insertion mutants to investigate transcript levels of sulfur metabolism related genes under standard conditions.
Project description:Col and hcr2 mutant from seedling and bud sample were harvested. Nuclei extracted using Honda buffer. Cross-linked chromatin was fragmented with mirococcal nuclease (MNase, NEB M0247S). After fragmentation, fragmented chromatin was incubated with H3K4me3 antibody (AbCam ab8580). TruSeq Prep Kit v2 (Illumina) were used for library construction.