Project description:We conducted transcriptome analysis of Komagataeibacter europaeus CGMCC 20445 samples under different acidity conditions to elucidate the changes in differentially expressed genes throughout the fermentation process.
Project description:In order to establish a profile of tumour vascular expression which could potentially be targeted therapeutically, we performed agilient microarray analysis on 4 samples each or renal cell carcinoma, colorectal cancer, colorectal metastasis to the liver and matched healthy tissues. The tumours had recieved no treatement prior to resection. The expression profiles were compared by one colour microarray analysis between matched healthy and tumour tissue. Genes expressed in all/or most of the comparisons were taken forward for further analysis. See acompanying publication: Wragg JW, Finnity JP, Anderson JA, Ferguson HJM, Bhatt RI, Murray PG, Heath VL, Bicknell R. MCAM is a vascular target in renal cancer. Cancer Research. 2016. The endothelum was isolated using collagenase V digestion, and dynabead magnetic bead isolation conjugated to lectin from Ulex Europaeus (Ulex Europaeus Agglutinin I (UEA I)), which binds human endothelial cells.
