Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of hESCs overexpressing inducible KLF17 versus uninduced controls.

ABSTRACT: RNA-seq of hESCs overexpressing inducible KLF17 versus uninduced controls.

PROVIDER: PRJEB47663 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of hESCs overexpressing inducible KLF17 versus uninduced controls.

Project description:Bulk RNA-seq of H9 human embryonic stem cells engineered to express a Dox-inducible KLF17_HA transgene. Uninduced (UI) and induced (+Dox) samples were collected each day between Day 0 (pre-induction) and Day 5 (post-Dox induction for +Dox or standard growth conditions for UI). Cells were continually cultured in conventional mTeSR1 hESC medium (StemCell Tech).

2021-10-02 | E-MTAB-10958 | biostudies-arrayexpress

E-MTAB-10958

Project description:RNA-seq of hESCs overexpressing inducible KLF17 versus uninduced controls.

2024-03-04 | E-MTAB-10958 | ExpressionAtlas

Comparison non-targeting hRNA hESCs versus UBE2K shRNA hESCs

Project description:Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. When compared with their differentiated counterparts, immortal human embryonic stem cells (hESCs) have a unique chromatin architecture and low levels of trimethylated histone H3 at lysine 9 (H3K9me3), a heterochromatin-associated modification. Here we assess a link between the intrinsic epigenetic landscape and ubiquitin-proteasome system of hESCs. We find that hESCs exhibit high expression of UBE2K, a ubiquitin-conjugating enzyme. Loss of UBE2K increases the levels of H3K9 trimethyltransferase SETDB1, resulting in H3K9 trimethylation and repression of neurogenic genes during differentiation. Concomitantly, loss of UBE2K impairs the ability of hESCs to differentiate into neural progenitors with neurogenic properties. Besides H3K9 trimethylation, we find that UBE2K binds histone H3 to induce its polyubiquitination and degradation by the proteasome. Notably, ubc-20, the worm orthologue of UBE2K, also regulates both histone H3 levels and H3K9 trimethylation in C. elegans germline. Thus, our results indicate that UBE2K crosses evolutionary boundaries to promote histone H3 degradation and reduce H3K9me3 repressive marks in immortal cells.

2020-06-01 | PXD018625 | Pride

A673 inducible-rescue experiment

Project description:A673 Ewing's sarcoma cells, with inducible EWS/FLI cDNA, harboring the EF-2-RNAi retrovirus, induced (or uninduced) for the indicated time period. Keywords: time course

2006-05-15 | GSE4563 | GEO

Expression data from inducible ES stable cell line overexpressing specifically the human gene WBSCR1

Project description:In order to identify the effects of the induction of the gene of interest (WBSCR1) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing WBSCR1

2019-10-11 | GSE95269 | GEO

Global transcriptome analysis of WT versus HEB-/- hESCs

Project description:To examine genome-wide changes in mRNA expression, we performed RNA-Seq on HEB-/- and WT hESCs. There were 274 significant changes in mRNA expression (p<0.05) between HEB-/- and WT hESCs; 126 transcripts were lower and 148 transcripts were higher

2017-06-24 | GSE100417 | GEO

Using hESCs to Probe the Interaction of CDKAL1 and MT1E, Two GWAS identified Diabetes Associated Genes

Project description:We report the RNA expression of insulin-GFP+ cells derived from CDKAL1-/- hESCs and CDKAL1-/-hESCs overexpressing MT1E

2017-07-01 | GSE97591 | GEO

Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2I

Project description:In order to identify the effects of the induction of the gene of interest (GTF2I) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing the human isorofm of the gene GTF2I

2019-10-11 | GSE95270 | GEO

Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2IRD2

Project description:In order to identify the effects of the induction of the gene of interest (GTF2IRD2) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing the human isorofm of the gene GTF2IRD2

2019-10-11 | GSE95268 | GEO

Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2IRD1

Project description:In order to identify the effects of the induction of the gene of interest (GTF2IRD1) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing the human isorofm of the gene GTF2IRD1.

2019-10-11 | GSE95267 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data