Project description:The aim of the project is to identify transcriptome-wide binding sites for the global RNA-binding protein ProQ in Salmonella during intracellular-like conditions.
Project description:This project explores the Arg-GlcNAcylation on Salmonella proteins GlmR and NagC. Using MS analysis and Site directed mutagenesis we show Arg-GlcNAcylation occurs on these salmonella enzymes.
Project description:Affymetrix 10K SNP mapping arrays were used to profile 14 basal cell carcinomas (BCCs) with matched blood DNA samples. Loss of heterozygosity (LOH) and copy number abnormality (CNA) profiles were derived from each tumour-blood pair. Keywords: Genomic DNA on Affymetrix 10K SNP array
Project description:This project explores the impact of SseK mediated Arg-GlcNAcylation on Salmonella proteins. Using Site directed mutagenesis, MS analysis and phenotypic studies we show Arg-GlcNAcylation appears to modulate the functions of multiple salmonella enzymes. This work demonstrates effectors can have multiple roles both within host cells and bacterial pathogens themselves.
