Eimeria acervulina genome
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Project description:In a collaboration between Welcome Trust Sanger Institute (WTSI), Royal Veterinary College (RVC) and King Abdullah University of Science and Technology (KAUST), we have sequenced, assembled and annotated the genome of Eimeria necatrix Houghton.
| PRJEB4833 | ENA
Project description:In a collaboration between Welcome Trust Sanger Institute (WTSI), Royal Veterinary College (RVC) and King Abdullah University of Science and Technology (KAUST), we have sequenced, assembled and annotated the genome of Eimeria praecox Houghton.
| PRJEB4836 | ENA
Project description:In a collaboration between Wellcome Trust Sanger Institute (WTSI), Royal Veterinary College (RVC) and King Abdullah University of Science and Technology (KAUST), we have sequenced, assembled and annotated the genome of Eimeria mitis Houghton.
| PRJEB4835 | ENA
Project description:In a collaboration between Wellcome Trust Sanger Institute (WTSI), Royal Veterinary College (RVC) and King Abdullah University of Science and Technology (KAUST), we have sequenced, assembled and annotated the genome of Eimeria maxima Weybridge.
| PRJEB4864 | ENA
Project description:In a collaboration between Welcome Trust Sanger Institute (WTSI), Royal Veterinary College (RVC) and King Abdullah University of Science and Technology (KAUST), we have sequenced, assembled and annotated the genome of Eimeria brunetti Houghton.
| PRJEB4834 | ENA
Project description:Enterotoxigeneic Escherichia coli (ETEC) is a leading cause of diarrhoeal infections in young children living in endemic regions in low and middle-income countries and adults travelling to these destinations. CFA/I fimbriae have been identified as the predominant colonisation factor associated with human ETEC infections. Here we used used Transposon-directed insertion-site sequencing (TraDIS) and transcriptomic analysis to identify the essential genome of the prototypical CFA/I expressing ETEC strain H10407 and uncover the survival mechanisms that enhance persistence of ETEC isolates in water and within mammalian hosts. RNA transcription profiles of H10407 were identified under different in vitro growth conditions including aerobic growth in neutral LB media (pH7); aerobic growth in acidic media (pH5); aerobic growth in alkaline media (pH9); anaerobic growth in neutral LB media (pH7); and survival in fresh water. Research work including RNA preparation and bioinformatics and statistical analyses were conducted at the Wellcome Trust Sanger Institute (WTSI) and the Westmead Institute for Medical Research (WIMR), the University of Sydney. The sequencing data was generated in the Bioscience Core Laboratory at King Abdullah University of Science and Technology (KAUST).
2021-04-21 | E-MTAB-9364 | biostudies-arrayexpress
Project description:The DMDD Programme (Deciphering the Mechanisms of Developmental Disorders) provides a free online database of morphological and molecular phenotypes from embryonic-lethal mouse gene knockouts (http://www.dmdd.org.uk/). Embryos are imaged using HREM, placentas are examined by histology and mutant embryo mRNA expression profiles are compared to wild type. Total RNA was extracted from somite number staged homozygous mutant, heterozygous and sibling wild-type E9.5 whole C57BL/6N embryos and DNase treated. The embryos were derived from Mouse Genetics Programme gene knockout lines defined as homozygous embryonic lethal (http://www.sanger.ac.uk/science/collaboration/mouse-resource-portal). Stranded RNA-seq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.<br> Notes about samples and libraries: </br><br>(1) The knockout alleles in this project were: Cnot4: Cnot4^tm1b(EUCOMM)Wtsi ; Ssr2: Ssr2^tm1b(EUCOMM)Wtsi ; 4933434E20Rik: 4933434E20Rik^tm1a(EUCOMM)Wtsi ; 1700007K13Rik: 1700007K13Rik^tm2b(EUCOMM)Wtsi ; Camsap3: Camsap3^tm1a(EUCOMM)Wtsi ; Anks6: Anks6^tm1b(KOMP)Wtsi ; Pdzk1: Pdzk1^tm2b(EUCOMM)Wtsi ; Otud7b: Otud7b^tm1b(EUCOMM)Wtsi ; Adamts3: Adamts3^tm1b(KOMP)Wtsi; Mir96: Mir96^tm2.2WTSI ; Cyp11a1: Cyp11a1^tm1b(EUCOMM)Hmgu and Traf6: Traf6^tm2a(EUCOMM)Wtsi. </br><br> (2) A combination of litter identifier and embryo identifier within a litter will unambiguously identify a single embryo used in this study. </br><br> (3) There is a margin of error for the somite-stage information (+/- 1 somite) because some embryos could be in between somite stages.</br><br> (4) There are a minimum of three biological replicates per line. </br><br> (5) Sex of the embryos was determined post-RNA-seq by looking at the expression of Xist (strong expression in females only). </br><br> (6) Libraries were constructed in batches comprising multiple litters from one line from RNA-extraction to sequencing.</br><br> This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ .</br>
2015-02-11 | E-MTAB-5224 | biostudies-arrayexpress
Project description:The goal of this study is to compare gene expression changes in retinal degenerate Royal College of Surgeons (RCS) rats following an injection of human neural progenitor cells (hNPCs) using RNA-seq, as compared to RCS rats receiving a sham surgery and wild-type Long Evans rats. These changes may lead to understanding of the therapeutic potential of hNPCs in inducing photoreceptor survival and visual function preservation. RNA-seq from wild-type Long Evans rats, retinal degenerate Royal College of Surgeons (RCS) rats, and RCS rats following transplantation of human neural progenitor cells (hNPCs)
2016-05-16 | E-GEOD-70600 | biostudies-arrayexpress
Project description:Molecular mechanisms underlying retinal degeneration are not well characterized including in the widely utilized Royal College of Surgeons (RCS) rat model of retinal degeneration. To better understand molecular pathways driving retinal degeneration we performed mRNA transcriptomics on RCS retinas following the natural progression of the disease. This data can identify novel therapeutic targets for future development.
2024-02-28 | GSE237804 | GEO
Project description:The goal of this study is to compare gene expression changes in retinal degenerate Royal College of Surgeons (RCS) rats following an injection of human neural progenitor cells (hNPCs) using RNA-seq, as compared to RCS rats receiving a sham surgery and wild-type Long Evans rats. These changes may lead to understanding of the therapeutic potential of hNPCs in inducing photoreceptor survival and visual function preservation.
2016-05-16 | GSE70600 | GEO