Project description:We sequenced mRNA of adult males and females of Daphnia galeata from two clonal lineages (9 days old). Females had no eggs in their brood pouches to minimize expression from embryos and males were naturally produced without the addition of hormones or stressors.

2016-08-15 | E-MTAB-4981 | biostudies-arrayexpress

RNA-seq to investigate the effect of long term exposure to fish kairomones on gene expression in Daphnia galeata

Project description:Two Daphnia galeata clones showing very contrasting responses to fish kairomones were analysed in an RNAseq setup.

2019-01-31 | E-MTAB-6234 | biostudies-arrayexpress

Transcriptome Daphnia galeata

Project description:Transcriptome sequencing of Daphnia galeata and combined assembly

| PRJEB14950 | ENA

Population transcriptomics in Daphnia galeata

Project description:Population transcriptomics in Daphnia galeata

| PRJEB23352 | ENA

Gene expression profiles of Daphnia magna exposed to Cd at the 1/10 LC50

Project description:Cadmium (Cd) is a toxic metal causing sublethal and chronic effects in crustaceans. Omic technologies offer unprecedented opportunities to better understand modes of toxicity by providing a holistic view of the molecular changes underlying physiological disruption. We sought to use gene expression and metabolomic analyses to reveal the processes leading to chronic Cd toxicity in the indicator species, Daphnia magna, after a 24-h sublethal exposure (18 ug/L, corresponding to 1/10 LC50). We first confirmed that metabolites can be detected and identified in small volumes (~3-6 ul) of D. magna hemolymph using Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry and NMR spectroscopy. We then compared the altered metabolite levels from a mass spectrometry metabolomics study to differentially expressed genes identified by a D. magna 44k oligonucleotide microarray. Metabolomics identified several essential amino acids, nucleotides and fatty acids as decreased in D. magna hemolymph following Cd exposure. Transcriptional changes included decreased levels of digestive enzymes and increased expression of genes related to embryonic development. The integration of metabolomic and transcriptomic profiles, as well as incorporation of results from previous studies, has enabled construction of a conceptual model detailing how sublethal Cd disrupts energy reserves and reproduction resulting in chronic toxicity. Daphnia magna were exposed to 18 micrograms/L Cadmium sulfate for 24 hours. RNA was extracted and hybridized to a custom Daphnia magna microarray to determine genes differentially expressed by the treatment. Two treament experiment:Unexposed and Cd treatment, 6 replicates for each condition

2010-12-31 | E-GEOD-20218 | biostudies-arrayexpress