Red clover
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB49635 | ENA |
REPOSITORIES: ENA
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Project description:Transcriptomes analysis of the petals from a red-flowered white clover mutant (red flowers) and its shade-treated counterpart (white flowers) grown under shaded conditions was performed using high-throughput sequencing. We obtained 121,626,564 and 130,577,944 clean reads in red-flowered mutant and treated counterpart, respectively. Of these clean reads, we respectively gained 125,350 and 99,638 unigene sequencces in two groups. As a result, a total of 157,964 unigenes were obtained with an average length of 728 bp and a median length of 1346 bp. These findings provideed insights into the expression profiles in red-flowered white clover mutant, and deepened our understanding of flower pigmentation in white clower.
2018-12-04 | GSE101059 | GEO
Project description:Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolerance. Through this approach, we wanted to identify proteins that are associated with the improvement of freezing tolerance in red clover. Recurrent selection performed indoor is an effective approach to improve the freezing tolerance of red clover. Significant improvement of freezing tolerance by recurrent selection was associated with differential accumulation of a small number of cold-regulated proteins that may play an important role in the determination of the level of freezing tolerance.
2016-03-15 | PXD003689 | Pride
Project description:To investigate the mechanisms related to anti-ferroptotic effects of red clover extract, we performed differentially expressed genes analysis using data obtained from the RNA-seq of system xCT knockout mouse embryonic fibroblast cells.
2023-12-01 | GSE221119 | GEO
Project description:Assembly of red clover (Trifolium pratense L.) genome
| PRJEB9186 | ENA
Project description:White clover mosaic virus (WCMV) is a major pathogen of white clover (Trifolium repens L.), with significant effects on yield and persistence. Due to the absence of natural sources of WCMV resistance a transgenic strategy has been employed to produce plants constitutively expressing WCMV replicase gene derivatives, designed to inhibit the propagation of WCMV through an RNA silencing mechanism. A 12,000 feature oligonucleotide microarray has been used to identify global changes in host plant, in addition to virus genome-encoded gene expression associated with WCMV infection in non-transgenic and transgenic WCMV-resistant white clover. Pairwise comparison between the transcriptome of mock-inoculated non-transgenic and WCMV-inoculated transgenic plants provides clear evidence for substantial equivalence between these two genotype/treatments, and demonstrate the efficacy of the transgenic strategy. WCMV- inoculated non-transgenic plants exhibit elevated abundance of many virus-encoded, and host immune response-specific transcripts compared to the transgenic resistant plants or mock-inoculated non-transgenic plants. By contrast, relative to inoculated sensitive plants, the majority of significantly up-regulated genes in mock-inoculated non-transgenic plants or WCMV-inoculated transgenic plants are markers of healthy cellular function. These results, and the occurrence of levels of WCMV-encoded transcripts in inoculated transgenic plants equivalent to those in virus-free plants, confirm the validity of the transgenic RNA silencing approach.<br>
2010-06-30 | E-MEXP-1688 | biostudies-arrayexpress
Project description:In recent years, a new class of viral noncoding subgenomic (ncsg)RNA has been identified that is generated as a stable degradation product formed via an exonuclease-resistant (xr) RNA structure that blocks the progression of 5’-3’ exonuclease that degrades viral RNAs in infected cells. The xrRNA-derived ncsgRNAs have been implicated in virus pathogenicity, virus movement and transmission, and suppressing antiviral defense responses. Although ncsgRNAs of animal viruses have been studied extensively, research on the role of xrRNA-derived plant viral ncsgRNAs remain scarce. In this study, we assess the effects of the ncsgRNA of red clover necrotic mosaic virus (RCNMV), called SR1f, in infected plants. We demonstrate that the presence of SR1f leads to severe symptoms and increased viral RNA accumulation in Nicotiana benthamiana and Arabidopsis thaliana. We also provide evidence that suppression of RNA silencing is not the primary function of SR1f. Additionally, a comparative transcriptomic analysis in N. benthamiana infected with wildtype RCNMV and an SR1f-deficient mutant RCNMV reveal that wt RCNMV infection that produces SR1f has a greater and significant impact on cellular gene expression resulting in deregulation of several cellular pathways with upregulation of genes mainly involved in plant hormone signaling, plant-pathogen interaction, MAPK signaling, and several metabolic pathways and downregulation of genes involved mainly in photosynthesis.
2021-06-26 | GSE178909 | GEO
Project description:Red clover (Trifolium pratense L.) is one of the most important legume forage species in temperate livestock agriculture. Tetraploid red clover cultivars are generally producing less seed than diploid cultivars. Improving the seed setting potential of tetraploid cultivars is necessary in order to utilize the high forage quality and environmentally sustainable nitrogen fixation of red clover. Two genotypes, one from cv.Tripo with weak seed setting and one from cv.Lasang with strong seed setting, were selected based on data from field experiments for transcriptome analysis of developing flower buds. De novo and reference based analyses of transcriptome assemblies were conducted to study the global transcriptome changes from early to late developmental stages of flower development of the two contrasting red clover genotypes. Transcript profiles, gene ontology enrichment and KEGG pathway analysis indicate that genes related to flower development, pollen pistil interactions, photosynthesis and embryo development are differentially expressed between the Tripo and Lasang genotypes. A significant number of genes related to pollination was overrepresented in Lasang, which might be a reason for its good seed setting ability. The candidate genes detected in this study might be used to develop molecular tools for breeding tetraploid red clover varieties with improved seed yield potentials.
2017-01-31 | E-MTAB-5117 | biostudies-arrayexpress
Project description:SWATH-MS was used to compare relative protein quantities of bee origin in manuka and clover honey to royal jelly.
2023-02-12 | PXD038889 | Pride
Project description:Red light can affect a variety of responses in Arabidopsis. We characterize the early gene expression patterns of roots exposed to 1 hour of red light. Early genes indicate elements involved in photomorphogenesis, chloroplast development, PAL pathways, root hair development are regulated by 1 hour of red light We used microarrays to detail the gene expression underlying the effects of red light on roots. Keywords: treatment
2006-08-15 | GSE4933 | GEO
Project description:Effects and role(s) of the noncoding subgenomic RNA of red clover necrotic mosaic virus in virus infection
| PRJNA741487 | ENA