Ontology highlight
ABSTRACT:
PROVIDER: PRJEB51539 | ENA |
REPOSITORIES: ENA
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Project description:In light of global reef decline new methods to accurately, cheaply, and quickly evaluate coral metabolic states are needed to assess reef health. Metabolomic profiling can describe the response of individuals to disturbance (i.e., shifts in environmental conditions) across biological models and is a powerful approach for characterizing and comparing coral metabolism. For the first time, we assess the utility of a proton-nuclear magnetic resonance spectroscopy (1H-NMR)-based metabolomics approach in characterizing coral metabolite profiles by 1) investigating technical, intra-, and inter-sample variation, 2) evaluating the ability to recover targeted metabolite spikes, and 3) assessing the potential for this method to differentiate among coral species. Our results indicate 1H-NMR profiling of Porites compressa corals is highly reproducible and exhibits low levels of variability within and among colonies. The spiking experiments validate the sensitivity of our methods and showcase the capacity of orthogonal partial least squares discriminate analysis (OPLS-DA) to distinguish between profiles spiked with varying metabolite concentrations (0 mM, 0.1 mM, and 10 mM). Finally, 1H-NMR metabolomics coupled with OPLS-DA, revealed species-specific patterns in metabolite profiles among four reef-building corals (Pocillopora damicornis, Porites lobata, Montipora aequituberculata, and Seriatopora hystrix). Collectively, these data indicate that 1H-NMR metabolomic techniques can profile reef-building coral metabolomes and have the potential to provide an integrated picture of the coral phenotype in response to environmental change.
2014-11-20 | MTBLS116 | MetaboLights
Project description:Multiple coral species including 2 Acropora spp, Montipora spp, Pocillopora acuta, Porites irregularis, Montipora aequituberculata, Porites lobata, Porites rus Raw sequence reads
| PRJNA325688 | ENA
Project description:Corals in nearshore marine environments are increasingly exposed to reduced water quality, which is the major local threat to coral reefs in Hawaii. Corals surviving in such conditions may have adapted to withstand sedimentation, pollutants, and other environmental stressors. Lobe coral (Porites lobata) populations from Maunalua Bay, Hawaii showed clear genetic differentiation along with distinct cellular protein expressions between the 'polluted, high-stress' nearshore site and the 'low-stress' offshore site. To understand the driving force of the observed genetic partitioning, reciprocal transplant and common-garden experiments were conducted using the nearshore and offshore colonies of P. lobata from Maunalua Bay to assess phenotypic differences between the two coral populations. Stress-related physiological and molecular responses were compared between the two populations. Proteomic responses highlighted the inherent differences in the cellular metabolic state and activities between the two populations under the same environmental conditions; nearshore corals did not significantly alter their proteome between the sites, while offshore corals responded to the nearshore transplantation with increased abundances of proteins associated with detoxification, antioxidant, and various metabolic processes. The response differences across multiple phenotypes suggest that the observed genetic partitioning was likely due to local adaptation.
2021-04-20 | PXD021407 | Pride
Project description:Bacterial Community Porites lobata coral mucus
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Project description:Pocillopora meandrina, genomic and transcriptomic data
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Project description:Porites lobata, genomic and transcriptomic data
| PRJEB51771 | ENA
Project description:Pocillopora damicornis, Pocillopora acuta, Pocillopora verrucosa, Pocillopora eydouxi, Pocillopora meandrina, Pocillopora ligulata, Pocillopora sp. B, Stylophora pistillata, Seriatopora hystrix Raw ezRAD sequence reads for species delineation
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Project description:Coral diazotroph communities - Pocillopora damicornis, Galaxea fascicularis, Porites rus, Acropora secale
| PRJNA668126 | ENA