Project description:In this work, we use RNAi and subsequent RNA isolation and Affymetrix Expression array analysis to map the genome-wide transcriptional targets of 107 of the strongest cell cycle regulators. Drosophila S2 cells were used with RNAi target gene knockdown compared to control (GFP dsRNA). RMA normalized data re-annotated using a custom CDF is available on the FTP site for this experiment. E-MTAB-1648, E-MTAB-1364 and E-MTAB-453 are all data from: Bonke M, et al. (2013) Transcriptional networks controlling the cell cycle. G3 (Bethesda) 3, 75-90, PMID: 23316440.

2012-06-30 | E-MTAB-453 | biostudies-arrayexpress

Pulmonary Suffusion in Controlling Minimal Residual Disease in Patients With Sarcoma or Colorectal Metastases

Project description:This phase I/II trial studies the side effects of pulmonary suffusion in controlling minimal residual disease in patients with sarcoma or colorectal carcinoma that has spread to the lungs. Pulmonary suffusion is a minimally invasive delivery of chemotherapeutic agents like cisplatin to lung tissues. Drugs used in chemotherapy, such as cisplatin, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. Pulmonary suffusion may also be useful in avoiding later use of drugs by vein that demonstrate no effect on tumors when delivered locally.

| 2306728 | ecrin-mdr-crc

Mechanisms Controlling Human Microglia Gene Expression

Project description:Mechanisms Controlling Human Microglia Gene Expression

| PRJNA388723 | ENA

PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency [ChIP-seq]

Project description:The Polycomb repressive system plays a fundamental role in controlling gene expression during mammalian development. To achieve this, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) bind target genes and use histone modification-dependent feedback mechanisms to form Polycomb chromatin domains and repress transcription. The interrelatedness of PRC1 and PRC2 activity at these sites has made it difficult to discover the specific components of Polycomb chromatin domains that drive gene repression and to understand mechanistically how this is achieved. Here, by exploiting rapid degron-based approaches and time-resolved genomics we kinetically dissect Polycomb-mediated repression and discover that PRC1 functions independently of PRC2 to counteract RNA polymerase II binding and transcription initiation. Using single-cell gene expression analysis, we reveal that PRC1 acts uniformly within the cell population, and that repression is achieved by controlling transcriptional burst frequency. These important new discoveries provide a mechanistic and conceptual framework for Polycomb-dependent transcriptional control.

2021-07-21 | GSE159398 | GEO

Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells [ChIP-Seq]

Project description:Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells

2020-05-06 | GSE135599 | GEO