IdPhyMeln1
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB52668 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Phyto melanocephala genome assembly, idPhyMeln1, alternate haplotype
| PRJEB52669 | ENA
Project description:Phyto melanocephala, genomic and transcriptomic data
| PRJEB51914 | ENA
Project description:The search for more effective methods to alleviate the negative effects of abiotic stresses in plants has motivated the experimentation of new technologies, namely nanotechnologies. It is in this context that the use of formulations containing hybrid silicon (Si) nanoparticles (NPs) and acting as delivery systems of the flavonoid quercetin was here investigated. These formulations, previously referred to as phyto-couriers, proved their efficacy in protecting textile hemp against salinity. We here broaden their application spectrum by studying the effects on an important crop model, tomato (Solanum lycopersicum cv. Micro-Tom). Two phyto-courier formulations, labelled GS1 and GS3, functionalized with 25 mg of quercetin and differing in the presence of trehalose were applied to salt-stressed plants (200 mM NaCl) by foliar spraying. Microscopy showed, under stress, a preservation of the palisade mesophyll cell structure in plants treated with the formulations. A trend towards decreased expression of some stress-responsive genes was observed in the leaves treated with the phyto-couriers. Shotgun proteomics confirmed the protective effects and the nano-biostimulant nature of the formulations, since several proteins involved in cytoprotection against oxidative stress were more abundant in control leaves treated with the pyto-courier. This was especially evident for the trehalose-containing GS3 formulation. Some proteins involved in chromatin remodelling were also more abundant in control leaves treated with GS3. Overall, the formulations showed promising results to enhance abiotic stress tolerance in a model crop through the mitigation of stress symptoms. The results presented are a proof-of-concept for the use of the phyto-courier nanotechnology in horticultural applications
2023-03-06 | PXD035460 | Pride
Project description:The naked mole-rat (NMR; Heterocephalus glaber) has recently gained considerable attention in the scientific community for its unique potential to unveil novel insights in the fields of medicine, biochemistry, and evolution. NMRs exhibit unique adaptations that include protracted fertility, cancer resistance, eusociality, and anoxia. This suite of adaptations is not found in other rodent species, suggesting that interrogating conserved and accelerated regions in the NMR genome will find regions of the NMR genome fundamental to their unique adaptations. However, the current NMR genome assembly has limits that make studying structural variations, heterozygosity, and non-coding adaptations challenging. We present a complete diploid naked-mole rat genome assembly by integrating long-read and 10X-linked read genome sequencing of a male NMR and its parents, and Hi-C sequencing in the NMR hypothalamus (N=2). Reads were identified as maternal, paternal or ambiguous (TrioCanu). We then polished genomes with Flye, Racon and Medaka. Assemblies were then scaffolded using the following tools in order: Scaff10X, Salsa2, 3d-DNA, Minimap2-alignment between assemblies, and the Juicebox Assembly Tools. We then subjected the assemblies to another round of polishing, including short-read polishing with Freebayes. We assembled the NMR mitochondrial genome with mitoVGP. Y chromosome contigs were identified by aligning male and female 10X linked reads to the paternal genome and finding male-biased contigs not present in the maternal genome. Contigs were assembled with publicly available male NMR Fibroblast Hi-C-seq data (SRR820318). Both assemblies have their sex chromosome haplotypes merged so that both assemblies have a high-quality X and Y chromosome. Finally, assemblies were evaluated with Quast, BUSCO, and Merqury, which all reported the base-pair quality and contiguity of both assemblies as high-quality. The assembly will next be annotated by Ensembl using public RNA-seq data from multiple tissues (SRP061363). Together, this assembly will provide a high-quality resource to the NMR and comparative genomics communities.
2022-06-27 | E-MTAB-11830 | biostudies-arrayexpress
Project description:The naked mole-rat (NMR; Heterocephalus glaber) has recently gained considerable attention in the scientific community for its unique potential to unveil novel insights in the fields of medicine, biochemistry, and evolution. NMRs exhibit unique adaptations that include protracted fertility, cancer resistance, eusociality, and anoxia. This suite of adaptations is not found in other rodent species, suggesting that interrogating conserved and accelerated regions in the NMR genome will find regions of the NMR genome fundamental to their unique adaptations. However, the current NMR genome assembly has limits that make studying structural variations, heterozygosity, and non-coding adaptations challenging. We present a complete diploid naked-mole rat genome assembly by integrating long-read and 10X-linked read genome sequencing of a male NMR and its parents, and Hi-C sequencing in the NMR hypothalamus (N=2). Reads were identified as maternal, paternal or ambiguous (TrioCanu). We then polished genomes with Flye, Racon and Medaka. Assemblies were then scaffolded using the following tools in order: Scaff10X, Salsa2, 3d-DNA, Minimap2-alignment between assemblies, and the Juicebox Assembly Tools. We then subjected the assemblies to another round of polishing, including short-read polishing with Freebayes. We assembled the NMR mitochondrial genome with mitoVGP. Y chromosome contigs were identified by aligning male and female 10X linked reads to the paternal genome and finding male-biased contigs not present in the maternal genome. Contigs were assembled with publicly available male NMR Fibroblast Hi-C-seq data (SRR820318). Both assemblies have their sex chromosome haplotypes merged so that both assemblies have a high-quality X and Y chromosome. Finally, assemblies were evaluated with Quast, BUSCO, and Merqury, which all reported the base-pair quality and contiguity of both assemblies as high-quality. The assembly will next be annotated by Ensembl using public RNA-seq data from multiple tissues (SRP061363). Together, this assembly will provide a high-quality resource to the NMR and comparative genomics communities.
2022-06-27 | E-MTAB-11831 | biostudies-arrayexpress
Project description:Porcine 60K BeadChip genotyping arrays (Illumina) are increasingly being applied in pig genomics to validate SNPs identified by re-sequencing or assembly-versus-assembly method. Here we report that more than 98% SNPs identified from the porcine 60K BeadChip genotyping array (Illumina) were consistent with the SNPs identified from the assembly-based method. This result demonstrates that whole-genome de novo assembly is a reliable approach to deriving accurate maps of SNPs.
2016-07-05 | GSE83910 | GEO