Project description:The aim of this experiment was to identify transcripts upregulated during vernalization in Lolium perenne plants. Illumina Genome Analyzer II RNA-Seq data was generated from leaf samples collected before vernalization, and after the start of cold treatment, at 2 days, 4 weeks and 9 weeks.
Project description:Falster is a Danish perennial ryegrass ecotype with strong vernalization requirement, while Veyo is an Italian variety with no requirement for vernalization in order to flower. The transcriptome of these two perennial ryegrass genotypes with contrasting vernalization requirements was studied during primary (vernalization and short day conditions), and secondary induction (higher temperature and long day conditions) using an RNA-Seq approach, in order to reveal transcripts with expression profiles indicative of a role in floral induction, both in the promotion and repression of flowering.