Transcriptome analysis of pre-anthesis development in barley
Ontology highlight
ABSTRACT: Expression profiling of developing barley main shoot apices and leaves during the vegetative phase and early inflorescence development in response to the photoperiod and natural allelic variation at Ppd-H1
Project description:The timing of reproductive development determines spike architecture and thus yield in temperate grasses such as barley (Hordeum vulgare L.). Reproductive development in barley is controlled by the photoperiod response gene Ppd-H1 which accelerates flowering time under long-day (LD) conditions. A natural mutation in Ppd-H1 prevalent in spring barley causes a reduced photoperiod response, and thus, late flowering under LD. However, it is not very well understood how LD and Ppd-H1 control pre-anthesis development, and thus spike architecture and yield in barley. We performed a detailed morphological analysis of the pre-anthesis development in the spring barley cultivar Scarlett and its wild barley derived introgression line S42-IL107, carrying the photoperiod responsive Ppd-H1 allele. Characterization of shoot apex development in these genotypes indicated that floral transition and initiation of floral primordia occurred under LD (16h light/ 8h dark) and short day (SD, 8h light/ 16h dark) conditions, while inflorescence and seed development strictly required LDs. Additionally, a fast photoperiod response in the presence of the dominant Ppd-H1 allele promoted floret fertility under LDs. To characterize the effects of the photoperiod and allelic variation at Ppd-H1 on gene expression during pre-anthesis development we performed RNA sequencing of leaves and developing main shoot apices during the vegetative phase and early stages of inflorescence development in Scarlett and S42-IL107 grown under SD and LD conditions. Main shoot apices of both genotypes were sampled at defined developmental stages, i.e. Waddington stage W0.5, W1.0, W2.0 and W3.5, respectively. Leaf samples were harvested from plants before (W1.0) and after floral transition (W2.0). We identified genes that were specifically regulated at floral transition independent of day-length and Ppd-H1 and thus may serve as markers for the staging of floral transition. Furthermore, we identified transcripts differentially expressed between photoperiods and between genotypes in leaves and in shoot apices. This set of transcripts might act as candidates downstream of Ppd-H1 and are correlated with the promotion of shoot apex development and higher floret fertility under LD and in the presence of the photoperiod responsive Ppd-H1 allele.
Project description:Expression profiling of developing barley main shoot apices and leaves during the vegetative phase and early inflorescence development in response to the photoperiod and natural allelic variation at Ppd-H1
Project description:Transcriptional profiling of inflorescence apices from wild type and rtr-2 mutant plants . Goal was to determine the differentially expressed genes between wild type and mutant plants in inflorescence apices.
Project description:Natural variation in the barley homolog of CENTRORADIALIS (HvCEN), was found to contribute to the expansion of barley cultivation into diverse habitats. It has been shown that induced hvcen mutants, originally designated as praematurum-c/maturity-c (mat-c) mutants, flowered a few days earlier under natural long-day conditions. All hvcen mutants flowered early and showed a reduction in spikelet number per spike, tiller number and yield in the outdoor experiments. Further evaluating development of main shoot apex of hvcen mutants and wild type under controlled long day and short day conditions showed that mutations in hvcen accelerated spikelet initiation and reduced axillary bud number in a photoperiod independent manner, but promoted floret development only under long days. In this project we investigate the pleiotropic effects of HvCEN on developmental timing and shoot and spike morphologies of barley and dependence on these effects on photoperiod. This RNAseq dataset was generated to identify the putative transcriptional targets of HvCEN. To this end, we used global transcriptome profiling in developing shoot apices and inflorescences of two allelic hvcen mutants (mat-c.907 and mat-c.943) and wild-type (Bonus) plants grown under long- and short-day photoperiods.
Project description:In the present study, we elucidate the molecular and hormonal role of the Six-rowed spike 2 (Vrs2) — a SHORT INTERNODES (SHI) transcriptional regulator during barley inflorescence and shoot development. Here we show that Vrs2 is specifically involved in floral organ patterning and phase duration by maintaining hormonal homeostasis and gradients during normal spike development; but similarly influenced plant stature traits. Furthermore, we establish a first link between the SHI-protein family and sucrose metabolism during organ growth and development, which may have implications for deeper molecular insights into crops' inflorescence and plant architecture. Differential gene expression study between BW-NIL(vrs2.e) vs. Bowman was done on the extracted RNA from immature shoot apices to infer the differences at level of expression at early spike developmental stages (triple mound (TM), glume primordia (GP), stamen primordia (SP), awn primordia (AP)).
Project description:Inflorescence apices of hec1,2,3 triple mutants and ethanol induced 35S:AlcR, AlcA:HEC1 plants were copared to wild-type or ethanol induced 35S:AlcR, AlcA:GUS apices, respectively.
Project description:RNA was isolated from vegetative apices of 7d-old short-day grown plants. Overexpression of miR164b and miR172a versus control. Keywords: parallel sample
