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RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

ABSTRACT: RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

PROVIDER: PRJEB8849 | ENA |

REPOSITORIES: ENA

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RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

Project description:The aim of this experiment was to observe the transcriptional profile of individual populations of cells in mouse islets throughout development. MARIS was used to isolate Ins+ islet cells at E18.5 and P13, and further separate Ucn3- and Ucn3+ populations at the transition point P4 and P5.

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Project description:We've developed a new Method to Analyze RNA following Intracellular Sorting (MARIS) allowing us to carry out gene expression studies on cells sorted based on intracellular immunoflourescence. The purpose of this study is to determine the degree of bias that MARIS introduces on gene expression. We report Illumina microarray gene expression data from human embryonic stem cells differentiated to a stage in which insulin-expressing cells are present. Gene expression data using RNA isolated from live cells is compared to gene expression data using RNA isolated from MARIS processed cells (fixed, permeabilized, antibody stained and mock sorted) to determine the degree of correlation in gene expression between these two biologically identical samples.

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