Genomics

Dataset Information

294


ABSTRACT: Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis

PROVIDER: PRJEB9516 | ENA |

REPOSITORIES: ENA

altmetric image

Publications

Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.

Kang Jinsuk J   Lienhard Matthias M   Pastor William A WA   Chawla Ashu A   Novotny Mark M   Tsagaratou Ageliki A   Lasken Roger S RS   Thompson Elizabeth C EC   Surani M Azim MA   Koralov Sergei B SB   Kalantry Sundeep S   Chavez Lukas L   Rao Anjana A  

Proceedings of the National Academy of Sciences of the United States of America 20150721 31


Dioxygenases of the TET (Ten-Eleven Translocation) family produce oxidized methylcytosines, intermediates in DNA demethylation, as well as new epigenetic marks. Here we show data suggesting that TET proteins maintain the consistency of gene transcription. Embryos lacking Tet1 and Tet3 (Tet1/3 DKO) displayed a strong loss of 5-hydroxymethylcytosine (5hmC) and a concurrent increase in 5-methylcytosine (5mC) at the eight-cell stage. Single cells from eight-cell embryos and individual embryonic day  ...[more]

Similar Datasets

2015-04-02 | PXD001857 | Pride
2014-09-11 | E-GEOD-56650 | ArrayExpress
| GSE107204 | GEO
| GSE66833 | GEO
| GSE66832 | GEO
2016-09-01 | E-GEOD-60777 | ArrayExpress
2014-04-17 | E-GEOD-55049 | ArrayExpress
| GSE85674 | GEO
| GSE85743 | GEO
2013-02-01 | E-GEOD-42396 | ArrayExpress