Project description:RATIONALE: Studying the genes expressed in samples of tissue from patients with cancer may help doctors identify biomarkers related to cancer.
PURPOSE: This laboratory study is using gene expression profiling to evaluate normal tissue and tumor tissue from patients with colon cancer that has spread to the liver, lungs, or peritoneum.
Project description:We generated the individual transcriptomes of 96 liver cells using the Fluidigm C1 platform. In brief, a suspension of cells was prepared from the liver of a 14-week old B6CastF1 (C57Bl/6J mother x CAST/Ei father) female mouse and loaded onto a 10-17 m C1 Single-Cell Auto Prep IFC (Fluidigm), and cell capture was performed according to the manufacturers instructions.
