Project description:af47_thioredoxins - comparison ws vs de and dy - Knock-out mutants of the ferredoxin-thioredoxin reductase were used to evaluate the impact of the redox perturbation of the plastidial thioredoxins on Arabidopsis transcriptome. - Wild-type (WS) and two T-DNA mutant lines for the variable subunit of ferredoxin:thioredoxin reductase ( DY and DE from INRA of Versailles collection) were compared Keywords: wt vs mutant comparison
Project description:The aim of this study was to analyze the impact of autotetraploidy on gene expression in Arabidopsis thaliana by comparing diploid versus tetraploid transcriptomes. In particular, this included the comparison of the transcriptome of different tetraploid A. thaliana ecotypes (Col-0 vs. Ler-0). The study was extended to address further aspects. One was the comparison of the transcriptomes in subsequent generations. This intended to obtain information on the genome wide stability of autotetraploid gene expression. Another line of work compared the transcriptomes of different diploid vs. tetraploid tissues. This aimed to investigate whether particular gene groups are specifically affected during the development of A. thaliana autotetraploids. Samples 1-8: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Col-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 9-12: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Ler-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 13-24: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Col-0 leaves (6th - 8th). The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 25-32: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Ler-0 leaves (6th - 8th). The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 33-36: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid vs. tetraploid Ler-0 seedlings from the second (F2) and third (F3) generation after induction, respectively. The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 37-40: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid vs. tetraploid Col-0 seedlings from the second (F2) and third (F3) generation after induction, respectively. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 41-44: Arabidopsis thaliana Col-0/Ler-0 diploid transcriptome. Transcriptional profiling and comparison of diploid Col-0 vs. diploid Ler-0 seedlings. The experiment was carried out with pedigree of esrablished lines. Samples 45-48: Arabidopsis thaliana Col-0/Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid Col-0 vs tetraploid Ler-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 and Ler-0 lines.
Project description:adt08-01_upp - adt08-01_upp - Impact on gene expression of the UPP loss-of-function at early development stage - WT/upp comaparison at the beginning of the development alteration (4d for Col0/upp-1, 5d for WS/upp-2), and 3 days later (7d for Col0/upp-1, 8d for WS/upp-2) Keywords: wt vs mutant comparison
Project description:af31_haf2_gcn5 - comparison ws vs haf2_gcn5 - Genes whose expression during seed germination is affected by the mutation of two histone acetyltransferase genes GCN5 and HAF2 (the later encodes TAF1). - mRNA extraxted from 72h germinating seed of the mutants gcn5 and haf2 were used for comparison with Ws seed at the same satge. 2 repetitions techniques + 1 repetition biologique. Keywords: wt vs mutant comparison
Project description:au15-02_trx - effect of ftr mutation on arabidopsis trancriptome - Recherche nouvelles fonctions pour le système TRX plastidial. - - Comparison Mut1 vs sauv1 at the same developmental stage (8-10 leaves) as Mut1 at the time of sampling - Comparison Mut1 vs sauv1 at the same age (11 weeks) as Mut1 at the time of sampling - Comparison Mut2 vs sauv2 at the same developmental stage (8-10 leaves) as Mut1 at the time of sampling - Comparison Mut2 vs sauv2 at the same age (9 weeks) as Mut1 at the time of sampling Mut1 = ftrb Sauv1 = Col-0 Mut2 = ftra1.a2 Sauv2 = WT segregant from crossing between ftra1 (in WS genetic background) and ftra2 (in Col background)