Caenorhabditis elegans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA101161 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The embryonic muscle transcriptome of Caenorhabditis elegans
| PRJNA101543 | ENA
Project description:Background: The force generating mechanism of muscle is evolutionarily ancient; the fundamental structural and functional components of the sarcomere are common to motile animals throughout phylogeny. Recent evidence suggests that the transcription factors that regulate muscle development are also conserved. Thus, a comprehensive description of muscle gene expression in a simple model organism should define a basic muscle transcriptome that is also expressed in animals with more complex body plans. To this end, we have applied Micro-Array Profiling of Caenorhabditis elegans Cells (MAPCeL) to muscle cell populations extracted from developing Caenorhabditis elegans embryos. Results: Fluorescence Activated Cell Sorting (FACS) was used to isolate myo-3::GFP-positive muscle cells, and their cultured derivatives, from dissociated early Caenorhabditis elegans embryos. Microarray analysis identified 6,693 expressed genes, 1,305 of which are enriched in the myo-3::GFP positive cell population relative to the average embryonic cell. The muscle-enriched gene set was validated by comparisons to known muscle markers, independently derived expression data, and GFP reporters in transgenic strains. These results confirm the utility of MAPCeL for cell type-specific expression profiling and reveal that 60% of these transcripts have human homologs. Conclusions: This study provides a comprehensive description of gene expression in developing Caenorhabditis elegans embryonic muscle cells. The finding that over half of these muscle-enriched transcripts encode proteins with human homologs suggests that mutant analysis of these genes in Caenorhabditis elegans could reveal evolutionarily conserved models of muscle gene function with ready application to human muscle pathologies. Keywords: embryonic muscle, myo-3::GFP
2007-07-13 | GSE8462 | GEO
Project description:C. elegans embryonic timecourse in wt and mutant embryos
| PRJNA91231 | ENA
Project description:A gene expression fingerprint of C. elegans embryonic motor neurons.
| PRJNA101047 | ENA
Project description:Analysis of C. elegans muscle transcriptome using trans-splicing-based RNA tagging (SRT)
| PRJNA374711 | ENA
Project description:Deciphering the C. elegans embryonic transcriptome with tissue, time, and alternative splicing resolution
| PRJNA477006 | ENA
Project description:RNA-seq of FACS-sorted C. elegans embryonic cells
| PRJNA295677 | ENA
Project description:The nematode Caenorhabditis elegans has evolutionarily conserved EV signaling pathways. In this study, we apply a recently published method for high specificity purification of EVs from C. elegans to carry out target-independent proteomic and RNA analysis of EVs from C. elegans. Our experiments uncovered diverse coding and non-coding RNA transcripts as well as protein cargo types commonly found in human EVs.
2020-08-05 | GSE146973 | GEO