Dataset Information


Danio rerio

ABSTRACT: Identification of mesendoderm precursor- and neurectoderm precursor-enriched genes in Zebrafish embryos

ORGANISM(S): Danio rerio  



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Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification.

Brown Jamie L JL   Snir Mirit M   Noushmehr Houtan H   Kirby Martha M   Hong Sung-Kook SK   Elkahloun Abdel G AG   Feldman Benjamin B  

Proceedings of the National Academy of Sciences of the United States of America 20080821 34

A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This strategy enabled us to perform a genome-wide transcriptome comparison of germ layer precursor cells. Mesendoderm and ectoderm precursors cells isolated by this method differentiate  ...[more]

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