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ABSTRACT: Early gibberellin responses and DELLA protein direct targets in Arabidopsis

OTHER RELATED OMICS DATASETS IN: E-GEOD-8739

PROVIDER: PRJNA102081 | ENA |

REPOSITORIES: ENA

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Transcription profiling of Arabidopsis early gibberellin responses and DELLA protein direct targets (GEO Super Series)

Project description:This SuperSeries is composed of the following subset Series:; GSE8739: Early gibberellin responses in Arabidopsis; GSE8741: DELLA protein direct targets in Arabidopsis Experiment Overall Design: Refer to individual Series

2008-06-16 | E-GEOD-8785 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:DELLA protein direct targets in Arabidopsis

| PRJNA105285 | ENA

Transcription profiling of Arabidopsis early gibberellin responses

Project description:The aim is to identify early gibberellin responsive genes in a gibberellin deficient strain such as ga1-3. Such genes are likely regulated by DELLA proteins which are master gibberellin repressors. DELLA proteins are rapidly degraded after gibberellin treatment, but their direct target genes still need to be elucidated. Experiment Overall Design: A set of 4 biological replicates was generated for each treament. Arabidopsis seedlings were treated with water or 2 uM GA4 and whole shoots collected after 1h. A comparison of water vs. GA4 treated samples should render a list of early gibberellin responsive genes.

2008-06-16 | E-GEOD-8739 | biostudies-arrayexpress

Early gibberellin responses in Arabidopsis

2007-11-21 | GSE8739 | GEO

DELLA protein direct targets in Arabidopsis

2007-11-21 | GSE8741 | GEO

Transcription profiling of Arabidopsis transgenic lines with a gibberellin non responsive DELLA protein vs control to identify DELLA protein direct targets

Project description:The aim of this study is to identify early DELLA protein-responsive genes using a Dexamethasone (DEX)-inducible system. Two transgenic lines were used: one induces the expression of a dominant, gibberellin non-responsive DELLA protein (rga-delta17); the other is a control line that carries the same vector, but lacks the rga-delta17 transgene. By comparing the gene expression changes in the line that expresses the rga-delta17 protein in the presence or absence of DEX it is possible to identify putative targets of DELLA proteins. An empty vector transgenic line was included in this study to identify genes that might be regulated by the DEX inducible system that are not dependent on the DELLA protein. Experiment Overall Design: Seedlings of both transgenic lines were pretreated for 16 h with 2 uM GA4 to enhance gibberellin responses. Because DELLA proteins are strong signaling repressors, this pretreatment should maximize the effect of DELLA induction. Eight-day old seedlings were treated with 2 uM GA4 or a combination of 2 uM GA4 plus 10 uM DEX to induce the rga-delta17 transgene. Three biological replicas for the transgenic line that carries the DEX-inducible rga-delta17 transgene were generated at 2h and 4h. For the empty vector line, only 2 biological replicas were generated at 4h of treatment with 2 uM GA with or without 10 uM DEX.

2008-06-16 | E-GEOD-8741 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Early gibberellin responses in Arabidopsis

| PRJNA105283 | ENA

Genome-wide binding sites for RGA in Arabidopsis inflorescence apices

Project description:The Arabidopsis thaliana REPRESSOR OF GA gene (RGA) encodes a DELLA protein that associates with multiple transcription factors to control plant growth in response to the hormone gibberellin (GA) (1). As part of a screen for genes that mediate the function of RGA in stem growth and shoot meristem function, we performed ChIP-seq to identify genome-wide loci associated with RGA in inflorescence apices. To detect genes controlled by RGA in a gain-of-function semi-dwarf background, we used a GFP-tagged, gibberellin-insensitive version of RGA (RGAp:GFP-rga-delta17) (2). (1) J.-M. Daviere, P. Achard, Gibberellin signaling in plants. Development 140, 1147-1151 (2013). (2) A. Dill, T. P. Sun, Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics 159, 777-785 (2001).

2017-08-15 | GSE94926 | GEO

Transcription profiling of Arabidopsis seed and flowers to identify DELLA regulated transcripts

Project description:Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis

2006-10-01 | E-MEXP-849 | biostudies-arrayexpress

Arabidopsis mutants treated with flg22, Alternaria brassicicola, Methyl jasmonate and Pst DC3000 bacteria

Project description:Gibberellin (GA) promotes plant growth by destabilizing DELLA proteins. DELLA proteins integrate multiple hormonal and environmental stress responses. We investigated the role of GA and DELLA proteins in plant defence. We used microarrays to detail the global programme of gene expression controlled by DELLA proteins and identified distinct classes of differentially regulated genes in response to pathogens, hormones or pathogen elicitors.

2010-01-15 | GSE17464 | GEO

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