Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a cold-adapted fish species - Trematomus bernacchii - an Antarctic fish species. Keywords: Stress response Overall design: Three control fish are compared to 3 heat-shocked fish, 3 short-term recovery fish and 3 longer term recovery fish.
Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a cold-adapted fish (Trematomus bernacchii) and compare it to a temperate species (Gillichthys mirabilis). This SuperSeries is composed of the SubSeries listed below. Overall design: In the first experiment (Gillichthys mirabilis), three control fish are compared to three heat-shocked fish. In the second experiment (Trematomus bernacchii), three control fish are compared to 3 heat-shocked fish, 3 short-term recovery fish and 3 longer term recovery fish.
Project description:The Antarctic icefish, a family (Channichthyidae) of teleosts within the perciform suborder Notothenioidei, are the only known vertebrates without oxygen-transporting hemoglobins and that are largely devoid of circulating erythrocytes. To elucidate the evo-devo mechanisms underpinning the suppressed erythropoiesis in the icefish, we conducted comparative studies on the transcriptomes and microRNAomes of the primary hematopoietic tissues between an icefish (Chionodraco hamatus) and two red-blooded notothenioids (Trematomus bernacchii and Gymnodraco acuticeps). We identified substantial remodeling of the hematopoietic programs in the icefish through which erythropoiesis is selectively suppressed. Experimental verification showed that erythropoietic suppression in the icefish may be attributable to the upregulation of TGF-β signaling, which coincides with reductions in multiple transcription factors essential for erythropoiesis and the upregulation of hundreds of microRNAs, the majority (> 80%) of which potentially target erythropoiesis regulating factors. Overall design: Comparative transcriptome studies on 9 samples (hematopoietic tissues of three notothenioids fish)
| GSE70113 | GEO
Project description:Molecular and biochemical responsiveness toward climate change in the Antarctic fish Trematomus bernacchii