Project description:Genome-wide methylation profiling of mouse 3T3-L1 Cells comparing control wildtypes with cells stable transfected with slincRAD shRNA8 (sh8). Two-condition experiment, 3T3-L1 wildtype vs. slincRAD-shRNA8 stable transfected cells before and after Adipogenesis. One replicate per array.
Project description:Paral1 is a novel adipocyte-specific lincRNA upregulated during adipogenesis of 3T3-L1 cells. Knockdown of Paral1 by siRNA transfection in 3T3-L1 cells followed by transcriptomic analyses was performed.
Project description:Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed 3T3-L1 cells overexpressing Fbxl10 using retrovirus system containing LTR promoter were differentiated and RNA was extracted at day 2 of differentiation
