Project description:This SuperSeries is composed of the following subset Series: GSE4919: Translation State Array Analysis of Thrombin Stimulated Human Endothelial Cells GSE5352: Translation State Array Analysis of LPS Stimulated Human Endothelial Cells GSE5353: Translation State Array Analysis of Insulin Stimulated Human Endothelial Cells GSE5354: Translation State Array Analysis of TNF Stimulated Human Endothelial Cells GSE5385: Translation State Array Analysis of Histamine Stimulated Human Endothelial Cells GSE5386: Translation State Array Analysis of Serum Stimulated Human Endothelial Cells Refer to individual Series
Project description:Confluent human umbilical vein endothelial cells (HUVECs) were exposed to TNF-alpha (100 units/mL) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively-translated, mRNA species that were then used to probe cDNA arrays, a process known as Translation State Array Analysis (TSAA). Four samples were obtained from these experiments: control monosome, control polysome, TNF monosome and TNF polysome. using the normalized signal intensities from the GeneFilters, we calculated a translation index, or measure of movement of an mRNA molecule from the monosome to the polysome fraction upon stimulation. This calculation was made as follows: (TNF polysome/TNF monosome)/(control polysome/control monosome). Translational indices greater than 2.5 (upregulated) or lower than 0.4 (downregulated) were chosen for further study. Keywords: Translation State Array Analysis
2006-07-27 | GSE5354 | GEO
Project description:Translation State Array Analysis
