Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA104871 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:RNA co-immunoprecipitations with yeast She proteins
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Project description:Immunopurification results of cytoskeletal motor proteins in S. cerevisiae
| PRJNA152717 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE3968: Puf proteins, RNA co-immunopurification GSE3969: Puf3delta vs. wild type comparison Abstract: Genes encoding RNA-binding proteins are diverse and abundant in eukaryotic genomes. Although some have been shown to have roles in post-transcriptional regulation of the expression of specific genes, few of these proteins have been studied systematically. We have used an affinity tag to isolate each of the five members of the Puf family of RNA-binding proteins in Saccharomyces cerevisiae and DNA microarrays to comprehensively identify the associated mRNAs. Distinct groups of 40-220 different mRNAs with striking common themes in the functions and subcellular localization of the proteins they encode are associated with each of the five Puf proteins: Puf3p binds nearly exclusively to cytoplasmic mRNAs that encode mitochondrial proteins; Puf1p and Puf2p interact preferentially with mRNAs encoding membrane-associated proteins; Puf4p preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors; and Puf5p is associated with mRNAs encoding chromatin modifiers and components of the spindle pole body. We identified distinct sequence motifs in the 3'-untranslated regions of the mRNAs bound by Puf3p, Puf4p, and Puf5p. Three-hybrid assays confirmed the role of these motifs in specific RNA-protein interactions in vivo. The results suggest that combinatorial tagging of transcripts by specific RNA-binding proteins may be a general mechanism for coordinated control of the localization, translation, and decay of mRNAs and thus an integral part of the global gene expression program. Refer to individual Series
2006-03-07 | E-GEOD-4393 | biostudies-arrayexpress
Project description:zzYra1 co-IPed RNA vs. zzMex67 co-IPed RNA (zzYra1IP_v_zzMex67IP)
| PRJNA84471 | ENA
Project description:zzMex67 co-IPed RNA vs. zz co-IPed RNA (zzMex67IP_v_zzIP)
| PRJNA85491 | ENA
Project description:zzYra1 co-IPed RNA vs. zz co-IPed RNA (zzYra1IP_v_zzIP)
| PRJNA85493 | ENA
Project description:zzMex67 co-IPed RNA vs. Total RNA (zzMex67IP_v_TotalRNA)
| PRJNA84467 | ENA
Project description:zzYra1 co-IPed RNA vs. Total RNA (zzYra1IP_v_TotalRNA)
| PRJNA84469 | ENA
Project description:Trf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip)
| PRJNA122819 | ENA
Project description:RNA-coimmunopurifications with TAP-tagged Puf proteins from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control. Cells were grown to midlog phase and harvested by centrifugation. TAP-tagged Puf proteins were affinity purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input)and from purified protein samples by phenol-chloroform extraction. RNA samples were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/ dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast DNA microarrays over night at 65 degrees. For a detailed procedure see http://microarray-pubs.stanford.edu/yeast_puf and also Gerber AP et al. PLoS Biology, 2004.
2005-11-01 | E-SMDB-2622 | biostudies-arrayexpress