Project description:We generated gene expression profiles of 5 time points in murine lung development (E11.5, E13.5, E14.5, E16.5 and P5). The goal of this study was to establish a reference data set for exploration of large-scale similarities between transcriptomes in development and cancer. Experiment Overall Design: Whole lung expression profiles from C57BL/6J mice at E11.5 (pooled sample), E13.5, E14.5, E16.5 and P5.
Project description:In this study, time dependent genome wide lung mRNA profiling changes were assessed using C57BL/6J and A/J mice. Through comprehensive bioinformatics and functional genomics analyses, we identified both temporal and strain dependent gene expression patterns, systemically mapped key regulators, bioprocesses, and transcriptional networks controlling lung maturation, providing the basis for new therapeutic strategies to enhance lung function in preterm infants. In this study, we designed a genome-wide mRNA expression time-course study from E15.5 to Postnatal Day 0 (PND0) using lung RNAs from C57BL/6J and A/J mice that differ significantly in gestational length.
Project description:To better understand the temporal dynamics of gene expression during normal murine lung development we characterized global gene expression at 26 time points in three common inbred strains of mice (A/J, C57BL/6J, and C3H/HeJ). The data set provides a unique resource for identifying patterns of gene expression changes during normal lung development and for investigating the developmental origins of respiratory disease. The transcriptional profiles generated for lung development in three inbred strains of the laboratory mouse revealed concordance with pre-natal stages of lung development defined by anatomy and morphology. The genomic data support the view that the postnatal alveolar development is composed of 4 distinct molecular stages. The data revealed strain specific differences in the expression of genes related to respiratory cell differentiation, pulmonary innervation, metabolic pathway interactions, and immune system function. Mouse lungs or whole embryos (e09.5 only) were surgically dissected at 26 time points across all six canonical stages of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar-ALV, homeostatic-HOM) to identify transcriptional profiles associated with 9 molecular divisions of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar1-ALV1, alveolar2-ALV2, alveolar3-ALV3, alveolar4-ALV4, homeostatic-HOM). Lung tissue from three or four embryos collected at e11.5 or e12.5, respectively, was pooled for each sample to obtain sufficient RNA for array analysis. Tissue from at least three animals was collected for each time point (biological replicates). Only male mice were used in the study.
Project description:This dataset is populated with transcriptome data across embryonic and postnatal development from two standard mouse strains, C57BL/6J and DBA/2J. Users can evaluate expression profiles across cerebellar development in a deep time series.
Project description:To better understand the temporal dynamics of gene expression during normal murine lung development we characterized global gene expression at 26 time points in three common inbred strains of mice (A/J, C57BL/6J, and C3H/HeJ). The data set provides a unique resource for identifying patterns of gene expression changes during normal lung development and for investigating the developmental origins of respiratory disease. The transcriptional profiles generated for lung development in three inbred strains of the laboratory mouse revealed concordance with pre-natal stages of lung development defined by anatomy and morphology. The genomic data support the view that the postnatal alveolar development is composed of 4 distinct molecular stages. The data revealed strain specific differences in the expression of genes related to respiratory cell differentiation, pulmonary innervation, metabolic pathway interactions, and immune system function.