Project description:human peripheral lymphocytes were infected with the full virulent strain 141 or human avirulent Microtus strain 201, and their transcriptomes were determined and compared. The most impressive finding is that robust responses of lysosome, TLR signaling and FcR mediated phagocytosis pathways were provoked at 2 hpi in 201- but not in 141-infected lymphocytes, suggesting that human lymphocytes might be able to constrain infection caused by strain 201 but not 141.

2019-05-15 | GSE121084 | GEO

Host Transcriptomic responses to pneumonic plague reveal that Yersinia pestis inhibits both the initial adaptive and innate immune responses in mice

Project description:Pneumonic plague is the most deadly form of infection caused by Yersinia pestis and can progress extremely fast. However, our understanding on the host transcriptomic response to pneumonic plague is insufficient. Here, we used RNA-sequencing technology to analyze transcriptomic responses in mice infected with fully virulent strain 201 or EV76, a live attenuated vaccine strain lacking the pigmentation locus. Approximately 600 differentially expressed genes (DEGs) were detected in lungs from both 201- and EV76-infected mice at 12 hours post-infection (hpi). DEGs in lungs of 201-infected mice exceeded 2,000 at 48 hpi, accompanied by sustained large numbers of DEGs in the liver and spleen; however, limited DEGs were detected in those organs of EV-infected mice. Remarkably, DEGs in lungs were significantly enriched in critical immune responses pathways in EV76-infected but not 201-infected mice, including antigen processing and presentation, T cell receptor signaling among others. Pathological and bacterial load analyses confirmed the rapid systemic dissemination of 201-infection and the confined EV76-infection in lungs. Our results demonstrate that fully virulent Y. pestis strongly inhibits both the innate and adaptive immune responses that are substantially stimulated in a self-limited infection, which update our holistic views on the transcriptomic response to pneumonic plague.

2017-07-13 | GSE84341 | GEO

Yersinia pestis biovar Orientalis str. F1991016

Project description:Orientalis biovar of the causative agent of plague

| PRJNA17681 | ENA

Yersinia pestis biovar Antiqua str. E1979001

Project description:Antiqua biovar of the causative agent of plague

| PRJNA17683 | ENA

Yersinia pestis biovar Antiqua str. B42003004

Project description:Antiqua biovar of the causative agent of plague

| PRJNA17685 | ENA